Inteins comprise a large family of phylogenetically widespread self-splicing protein catalysts
that colonize diverse host proteins. The evolutionary and functional relationship between the …

Protein trans-splicing using split inteins is a powerful and convenient reaction to chemically
modify recombinantly expressed proteins under mild conditions. In particular, semisynthetic …

Inteins are proteins that can mediate protein-splicing reactions. The reaction is a
posttranslational modification where the intein sequence is excised from a protein precursor …

Split inteins are indispensable tools for protein engineering because their ligation and
cleavage reactions enable unique modifications of the polypeptide backbone. Three …

Split intein-mediated protein trans-splicing (PTS) is widely applied in chemical biology and
biotechnology to carry out traceless and specific protein ligation. However, the external …

A split‐intein consists of two complementary fragments (N‐intein and C‐intein) that can
associate to carry out protein trans‐splicing. The Ssp GyrB S11 split‐intein is an engineered …

Protein splicing mediated by inteins is a self-processive reaction leading to the excision of
the internal intein domain from a precursor protein and the concomitant ligation of the …

Inteins are internal protein segments embedded in various host proteins and are excised by
a post# translational process called protein splicing (Perler et al. 1994). During protein …

A protein ligase, intein, mediates a protein-splicing reaction. It can be split into two
complementary fragments and reconstituted as a whole intein scaffold to perform protein …

Protein splicing is a self‐catalyzed process in which an internal protein domain (the intein) is
excised from its flanking sequences, linking them together with a canonical peptide bond …