Our knowledge of biological systems relies increasingly on the ability of quantifying and
imaging intracellular signals and events in living subjects. The development of novel …

Today, inteins have been extensively used in a wide variety of applications in biotechnology.
Split inteins make up a small sector of inteins discovered more recently. Split inteins are …

Protein splicing is a posttranslational processing event that releases an internal protein
sequence from a protein precursor. During the splicing process the internal protein …

The split inteins from the DnaE cyanobacterial family are efficient and versatile tools for
protein engineering and chemical biology applications. Their ultrafast splicing kinetics allow …

The judicious application of intein technologies to biological problems has resulted in
powerful tools for biomedical research. Inteins are intervening sequences that excise …

Inteins catalyze a post-translational modification known as protein splicing, where the intein
removes itself from a precursor protein and concomitantly ligates the flanking protein …

Intein‐based protein cleavages, if carried out in a controllable way, can be useful tools of
recombinant protein purification, ligation, and cyclization. However, existing methods using …

Inteins are protein splicing elements that mediate their excision from precursor proteins and
the joining of the flanking protein sequences (exteins). In this study, protein splicing was …

Background Protein trans-splicing by naturally occurring split DnaE inteins is used for
protein ligation of foreign peptide fragments. In order to widen biotechnological applications …

Protein splicing domains, also called inteins, have become a powerful biotechnological tool
for applications involving molecular biology and protein engineering. Early applications of …