In Situ Hybridization as a Method to Study the Regulation of Gene Expression in Paramecium

S CURTENAZ, J BEISSON - Journal of Eukaryotic Microbiology, 1996 - Wiley Online Library
A method for whole cell in situ hybridization in Paramecium was developed, which allowed
us to visualize both the cytoplasmic mRNA and the transcripts in the macronucleus, and to …

A method for the amplification of Paramecium micronuclear DNA by polymerase chain reaction and its application to the central repeats of Paramecium primaurelia G …

F Caron, F Ruiz - The Journal of protozoology, 1992 - europepmc.org
This paper describes a method which allows the amplification of Paramecium micronuclear
DNA. Amacronucleate cells are first obtained by an appropriate treatment with nocodazole …

Analysis of Paramecium macronuclear DNA using pulsed field gel electrophoresis

HL PHAN, J FORNEY… - The Journal of …, 1989 - Wiley Online Library
We have analyzed the macronuclear DNA of Paramecium tetraurelia using orthogonal‐field‐
altemation gel electrophoresis. The mean size of the linear macronuclear DNA molecules is …

Regulation of Macronuclear DNA Content in Paramecium tetraurelia

JD BERGER - The Journal of Protozoology, 1979 - Wiley Online Library
The amitotic division of the macronucleus of Paramecium tetraurelia produces daughter
macronuclei which frequently differ in DNA content. In wild‐type cells these differences are …

Micronuclear DNA from Paramecium tetraurelia: Serotype 51 A Gene Has Internally Eliminated Sequences

LB Preer, GUY HAMILTON… - The Journal of …, 1992 - Wiley Online Library
A method for the isolation of micronuclear DNA from Paramecium tetraurelia has been
developed. After cell lysis, a low speed centrifugation at 1,000 g is used to remove all of the …

RNA‐dependent DNA polymerase activity in Paramecium tetraurelia: what for?

MD Katinka - European journal of biochemistry, 1987 - Wiley Online Library
Protein extracts from the protozoan ciliate Paramecium tetraurelia revealed high levels of
RNA‐dependent DNA polymerase activity (reverse transcriptase). Stable and constant …

Macronuclear DNA organization and transcription in Paramecium primaurelia

C McTavish, J Sommerville - Chromosoma, 1980 - Springer
Macronuclear DNA was isolated from Paramecium primaurelia, stock 168. Although the
macronucleus is polyploid to the extent of 840C, in other respect the DNA appears to be …

Induction of specific macronuclear developmental mutations by microinjection of a cloned telomeric gene in Paramecium primaurelia.

E Meyer - Genes & development, 1992 - genesdev.cshlp.org
In Paramecium, the differentiation of a highly polyploid macronucleus from a diploid nucleus
is accompanied by an extensive reorganization of the genome, involving reduction in …

A repetitive DNA sequence in Paramecium macronuclei is related to the β subunit of G proteins

J Forney, K Rodkey - Nucleic acids research, 1992 - academic.oup.com
A repeated DNA sequence has been identified in the macronucleus of several Paramecium
species. In P. tetraurelia the repeat was identified in the subtelomeric region of four …

Microtubule dynamics and morphogenesis in Paramecium: II. Modelling of the conversion of a transient molecular signal into a morphogenetical process

M Laurent, A Fleury - European Journal of Protistology, 1996 - Elsevier
In Paramecium, the deployment of an acetylated microtubular network is restricted to a part
of the ventral cell surface, the A-paratene zone. Upon cell division, this microtubular network …