Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …

Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …

Abstract Background Recently, the CRISPR/Cas9 system has been widely used to precisely
edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation …

Abstract The CRISPR/Cas9-sgRNA system has been developed to mediate genome editing
and become a powerful tool for biological research. Employing the CRISPR/Cas9-sgRNA …

Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …

Plant virus-mediated sgRNA delivery and expression have great advantages; sgRNA
expression can rapidly expand and accumulate along with virus replication and movement …

Use of the clustered regularly interspaced short palindromic repeat (CRISPR)-associated
protein9 (Cas9) and Cpf1 systems in plants (Ma et al., 2016; Wang et al., 2017) involves …

Genome editing has been a long-term challenge for molecular biology research, particularly
for plants possess complex genome. The recently discovered Clustered Regularly …

CRISPR/Cas9 genome targeting systems have been applied to a variety of species.
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …

CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …