Background Normalizing through reference genes, or housekeeping genes, can make more
accurate and reliable results from reverse transcription real-time quantitative polymerase …

Accurate and reliable gene expression data from qPCR depends on stable reference gene
expression for potential gene functional analyses. In this study, 15 reference genes were …

Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …

Genetically modified (GM) cotton lines have been approved for commercialization and
widely cultivated in many countries, especially in China. As a step towards the development …

Selection of reference genes is an essential consideration to increase the precision and
quality of relative expression analysis by the quantitative RT-PCR method. The stability of …

Background Usually the reference genes used in gene expression analysis have been
chosen for their known or suspected housekeeping roles, however the variation observed in …

Quantitative reverse transcription PCR (qRT-PCR) is one of the most precise and widely
used methods of gene expression analysis. A necessary prerequisite of exact and reliable …

A suitable reference gene is an important prerequisite for guarantying accurate and reliable
results in qPCR analysis. Celery is one of the representative vegetable in Apiaceae and is …

Background With the availability of genome sequences, gene expression analysis of jute
has drawn considerable attention for understanding the regulatory mechanisms of fiber …

The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method
for gene expression analysis requires one or several reference gene (s) acting as …