Mode of AmyR binding to the CGGN8AGG sequence in the Aspergillus oryzae taaG2 promoter

T Ito, S Tani, T Itoh, N Tsukagoshi, M Kato… - Bioscience …, 2004 - jstage.jst.go.jp
AmyR is a transcriptional activator in Aspergillus spp. necessary for induction of the
amylolytic enzyme genes. It recognizes 50-CGGN8CGG-30 conserved in a number of the …

Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae

S Tada, Y Iimura, K Gomi, K Takahashi… - Agricultural and …, 1989 - academic.oup.com
Abstract The Taka-amylase A (TAA) gene was cloned from the genomic library of A. oryzae
using synthetic DNA oligomers as probes. The gene was located in a 3.7 Kbp EcoRl …

Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus …

S Tada, K Gomi, K Kitamoto, K Takahashi… - Molecular and General …, 1991 - Springer
Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae
R11340 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The …

Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae …

T Minetoki, C Kumagai, K Gomi, K Kitamoto… - Applied microbiology …, 1998 - Springer
The role of the conserved sequence region III in the promoter regions of the amylase-
encoding genes amyB, glaA and agdA of Aspergillus oryzae was examined. Introduction of …

An overview of the CCAAT-box binding factor in filamentous fungi: assembly, nuclear translocation, and transcriptional enhancement

M Kato - Bioscience, biotechnology, and biochemistry, 2005 - Taylor & Francis
Filamentous fungi are frequently used for the production of industrial enzymes, since they
produce a variety of enzymes including polysaccharide-degrading enzymes. Among the …

Distinct mechanism of activation of two transcription factors, AmyR and MalR, involved in amylolytic enzyme production in Aspergillus oryzae

K Suzuki, M Tanaka, Y Konno, T Ichikawa… - Applied microbiology …, 2015 - Springer
The production of amylolytic enzymes in Aspergillus oryzae is induced in the presence of
starch or maltose, and two Zn 2 Cys 6-type transcription factors, AmyR and MalR, are …

Characterization of the amdA-regulated aciA gene of Aspergillus nidulans

JA Saleeba, CS Cobbett, MJ Hynes - Molecular and General Genetics …, 1992 - Springer
The structure, function and regulation of the acetate inducible aciA gene of Aspergillus
nidulans was analysed. The aciA locus was mapped to chromosome 1 at a position where …

Deletion analysis of the Taka-amylase A gene promoter using a homologous transformation system in Aspergillus oryzae

K Tsuchiya, S Tada, K Gomi, K Kitamoto… - Bioscience …, 1992 - jstage.jst.go.jp
Materials and Methods Strains, plasmids, and media. A. oryzae M-2-3 (argB') was used as a
recipient strain for transformation.” Escherichia coli JM109 [rat/11,(lac-proAB), endAI …

Contribution Ratios of amyA, amyB, amyC Genes to High-Level α-Amylase Expression in Aspergillus oryzae

T Nemoto, J Maruyama… - Bioscience, biotechnology …, 2012 - academic.oup.com
Aspergillus oryzae strains express α-amylases abundantly, and the genome reference strain
RIB40 has three α-amylase genes (amyA, amyB, and amyC). However, there is no …

Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA …

T Minetoki, K Gomi, K Kitamoto… - Bioscience …, 1995 - academic.oup.com
In Aspergillus oryzae wild-type strains, the expression of the agdA gene encoding α-
glucosidase (AGL) is induced by maltose at the transcriptional level in a similar manner to …