Cloning of the α-amylase cDNA of Aspergillus shirousamii and its expression in Saccharomyces cerevisiae

I Shibuya, G Tamura, T Ishikawa… - Bioscience, biotechnology …, 1992 - jstage.jst.go.jp
Materials and Methods Strains, plasmids, and phages. A. shirousamii RIB2504 was used as
a donor strain to construct a cDNA library. E. coli strains HB101, 1 “JM109, 12'and …

Development of Aspergillus oryzae thiA promoter as a tool for molecular biological studies

J Shoji, J Maruyama, M Arioka… - FEMS microbiology …, 2005 - academic.oup.com
In filamentous fungi, the repertoire of promoters available for exogenous gene expression is
limited. Here, we report the development and application of the thiamine-regulatable thiA …

Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels

RJ Gouka, PJ Punt… - Applied and …, 1997 - Am Soc Microbiol
In this study we have analyzed the effects of a glucoamylase gene fusion on the mRNA
levels and protein levels for the human interleukin-6 gene (hil6) and the guar alpha …

Regulation of the amylolytic and (hemi-) cellulolytic genes in aspergilli

N Tsukagoshi, T Kobayashi, M Kato - The Journal of general and …, 2001 - jstage.jst.go.jp
Hap complex is suggested to enhance transcription by remodeling the chromatin structure.
The regulation of gene expression in filamentous fungi of industrial interest could follow …

The promoter of the glucoamylase-encoding gene of Aspergillus niger functions in Ustilago maydis

TL Smith, J Gaskell, RM Berka, M Yang, DJ Henner… - Gene, 1990 - Elsevier
Promoter sequences from the Aspergillus niger glucoamylase-encoding gene (glaA) were
linked to the bacterial hygromycin (Hy) phosphotransferase-encoding gene (hph) and this …

Gel mobility shift scanning of the acetate-inducible promoters fromNeurospora crassa reveals a common co-inducible DNA-binding protein

T Mizote, M Bibbins, PJ Sheffield… - Molecular and General …, 1996 - Springer
The promoter regions of four acetate-inducible genes of Neurospora crassa, acu-3, acu-5,
acu-8 and acu-9, have been sequenced. Using a scanning gel mobility shift assay particular …

Increase in glucoamylase productivity of Aspergillus awamori strain by combination of radiation mutagenesis and plasmid transformation methods

YP Vinetsky, AM Rozhkova, AS Sereda… - Applied Biochemistry …, 2010 - Springer
Increase in the expression level of amylolytic genes activator protein encoded by amyR
gene was shown to result in enhancement of glucoamylase productivity of A. awamori strain …

Endoplasmic reticulum stress leads to the selective transcriptional downregulation of the glucoamylase gene in Aspergillus niger

H Al‐Sheikh, AJ Watson, GA Lacey… - Molecular …, 2004 - Wiley Online Library
We describe a new endoplasmic reticulum (ER)‐associated stress response in the
filamentous fungus Aspergillus niger. The inhibition of protein folding within the ER leads to …

Identification of the Sites of Action for Regulatory Genes Controlling the amdS Gene of Aspergillus nidulans

MJ Hynes, CM Corrick, JM Kelly… - Molecular and cellular …, 1988 - Taylor & Francis
The amdS gene of Aspergillus nidulans, which encodes an acetamidase enzyme, is
positively regulated by the trans-acting genes amdR, facB, amdA, and areA. Sequence …

Molecular cloning and transcriptional analysis of the Aspergillus terreus gla1 gene encoding a glucoamylase

L Ventura, L Gonzalez-Candelas… - Applied and …, 1995 - Am Soc Microbiol
The Aspergillus terreus gla1 gene, coding for a glucoamylase, has been cloned by
heterologous hybridization. The gene is interrupted by four introns and encodes a protein …