Cloning and expression of the α–L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae

JM Crous, IS Pretorius, WH Van Zyl - Applied microbiology and …, 1996 - Springer
First-strand cDNA was prepared from mRNA of Aspergillus niger MRC11624 induced on oat
spelts xylan. Using the cDNA as a template, the α-L-arabinofuranosidase gene (abf B) was …

Aspergillus oryzae S2 AmyA amylase expression in Pichia pastoris: production, purification and novel properties

S Trabelsi, M Sahnoun, F Elgharbi, R Ameri… - Molecular biology …, 2019 - Springer
A synthetic cDNA-AmyA gene was cloned and successfully expressed in Pichia pastoris as
a His-tagged enzyme under the methanol inducible AOX1 promoter. High level of …

Cell wall α-1, 3-glucan prevents α-amylase adsorption onto fungal cell in submerged culture of Aspergillus oryzae

S Zhang, H Sato, S Ichinose, M Tanaka… - Journal of bioscience …, 2017 - Elsevier
We have previously reported that α-amylase (Taka-amylase A, TAA) activity disappears in
the later stage of submerged Aspergillus oryzae culture as a result of TAA adsorption onto …

Fungal catabolic gene regulation: Molecular genetic analysis of theamdS gene ofAspergillus nidulans

MA Davis, JM Kelly, MJ Hynes - Genetica, 1993 - Springer
Aspergillus nidulans is an excellent experimental organism for the study of gene regulation.
Genetic and molecular analyses of trans-acting and cis-acting mutations have revealed a …

Identification of functional cis-elements required for repression of the Taka-amylase A gene under secretion stress in Aspergillus oryzae

B Zhou, C Wang, B Wang, X Li, J Xiao, L Pan - Biotechnology letters, 2015 - Springer
The expression of secreted proteins in filamentous fungi is down-regulated by a
transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression …

In plants the alc gene expression system responds more rapidly following induction with acetaldehyde than with ethanol

BH Junker, C Chu, U Sonnewald, L Willmitzer… - Febs …, 2003 - Wiley Online Library
It has recently been proposed that acetaldehyde is the physiological inducer of the alc gene
system and hence indirectly the activator of the AlcA promoter in Aspergillus nidulans. Here …

Sugar response sequence in the promoter of a rice α-amylase gene serves as a transcriptional enhancer

CA Lu, EK Lim, SM Yu - Journal of Biological Chemistry, 1998 - ASBMB
Expression of α-amylase genes in both rice suspension cells and germinating embryos is
repressed by sugars and the mechanism involves transcriptional regulation. The promoter of …

The amdR product and a CCAAT-binding factor bind to adjacent, possibly overlapping DNA sequences in the promoter region of the Aspergillus nidulans amdS …

R van Heeswijck, MJ Hynes - Nucleic acids research, 1991 - academic.oup.com
The amdS gene of Aspergilius nidulans is regulated by a number of positively acting
regulatory genes which act additively and independently. Using gel mobility shift assays with …

Molecular cloning of the uaY regulatory gene of Aspergillus nidulans reveals a favoured region for DNA insertions

T Suárez, N Oestreicher, MA Peñalva… - Molecular and General …, 1991 - Springer
The cloning of the positive regulatory gene, uaY, which mediates uric acid induction of
enzymes and permeases of the purine degradation pathway in the fungus Aspergillus …

A mutation affecting amdS expression in Aspergillus nidulans contains a triplication of a cis-acting regulatory sequence

ME Katz, JA Saleeba, SI Sapats, MJ Hynes - Molecular and General …, 1990 - Springer
In Aspergillus nidulans expression of the acetamidase structural gene, amdS, is under the
control of at least four regulatory genes including the trans-acting amdA regulatory gene. A …