CRISPR/Cas-based diagnostics in agricultural applications
Pests and disease-causing pathogens frequently impede agricultural production. An early
and efficient diagnostic tool is crucial for effective disease management. Clustered regularly …
and efficient diagnostic tool is crucial for effective disease management. Clustered regularly …
Versatile applications of the CRISPR/Cas toolkit in plant pathology and disease management
MS Wheatley, Y Yang - Phytopathology®, 2021 - Am Phytopath Society
New tools and advanced technologies have played key roles in facilitating basic research in
plant pathology and practical approaches for disease management and crop health …
plant pathology and practical approaches for disease management and crop health …
CRISPR-based point-of-care plant disease diagnostics
The coupling of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas
RNA-programmable nucleases with nucleic acid detection platforms has brought radical …
RNA-programmable nucleases with nucleic acid detection platforms has brought radical …
Do CRISPR-based disease diagnosis methods qualify as point-of-care diagnostics for plant diseases?
Effective plant disease management calls for a rapid, precise, and a convenient field-
deployable method for early diagnosis of phytopathogens, especially in cases where visual …
deployable method for early diagnosis of phytopathogens, especially in cases where visual …
The engineered CRISPR‐Mb2Cas12a variant enables sensitive and fast nucleic acid‐based pathogens diagnostics in the field
J Jiao, Y Liu, M Yang, J Zheng, C Liu… - Plant Biotechnology …, 2023 - Wiley Online Library
Existing CRISPR/Cas12a‐based diagnostic platforms offer accurate and vigorous
monitoring of nucleic acid targets, but have the potential to be further optimized for more …
monitoring of nucleic acid targets, but have the potential to be further optimized for more …
State of the art in CRISPR/Cas system-based signal conversion and amplification applied in the field of food analysis
B Lu, Z Guo, K Zhong, T Osire, Y Sun, L Jiang - Trends in Food Science & …, 2023 - Elsevier
Background Food safety is a major global public health issue. The majority of traditional
culture-based methods have lengthy detection times, require sophisticated sample …
culture-based methods have lengthy detection times, require sophisticated sample …
CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety
Z Mao, R Chen, X Wang, Z Zhou, Y Peng, S Li… - Trends in Food Science …, 2022 - Elsevier
Background In the context of the current pandemic caused by the novel coronavirus,
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …
CRISPR applications in plant virology: virus resistance and beyond
NO Kalinina, A Khromov, AJ Love… - …, 2020 - Am Phytopath Society
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
genes (Cas) is a prokaryotic adaptive immune system which has been reprogrammed into a …
genes (Cas) is a prokaryotic adaptive immune system which has been reprogrammed into a …
Efficient, rapid, and sensitive detection of plant RNA viruses with one-pot RT-RPA–CRISPR/Cas12a assay
Most viruses that infect plants use RNA to carry their genomic information; timely and robust
detection methods are crucial for efficient control of these diverse pathogens. The RNA …
detection methods are crucial for efficient control of these diverse pathogens. The RNA …
CRISPR-Cas12a-based diagnostics of wheat fungal diseases
K Mu, X Ren, H Yang, T Zhang, W Yan… - Journal of Agricultural …, 2022 - ACS Publications
Fusarium head blight (FHB) of wheat, mainly caused by Fusarium graminearum (F.
graminearum) infection, reduces crop yield and contaminates grain with mycotoxins. We …
graminearum) infection, reduces crop yield and contaminates grain with mycotoxins. We …