SARS-CoV-2 whole-genome sequencing using Oxford nanopore technology for variant monitoring in wastewaters

L Barbé, J Schaeffer, A Besnard, S Jousse… - Frontiers in …, 2022 - frontiersin.org
Since the beginning of the Coronavirus Disease-19 (COVID-19) pandemic, multiple Severe
Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) mutations have been reported …

Genome sequencing of SARS-CoV-2 allows monitoring of variants of concern through wastewater

M Herold, AF d'Hérouël, P May, F Delogu… - Water, 2021 - mdpi.com
Monitoring SARS-CoV-2 in wastewater has shown to be an effective tool for epidemiological
surveillance. More specifically, RNA levels determined with RT-qPCR have been shown to …

Monitoring SARS-CoV-2 variants alterations in Nice neighborhoods by wastewater nanopore sequencing

G Rios, C Lacoux, V Leclercq, A Diamant… - The Lancet Regional …, 2021 - thelancet.com
Background Wastewater surveillance was proposed as an epidemiological tool to define the
prevalence and evolution of the SARS-CoV-2 epidemics. However, most implemented …

Key SARS-CoV-2 mutations of alpha, gamma, and eta variants detected in urban wastewaters in Italy by long-read amplicon sequencing based on nanopore …

G La Rosa, D Brandtner, P Mancini, C Veneri… - Water, 2021 - mdpi.com
The emergence of SARS-CoV-2 variants of concern (VOCs) and variants of interest (VOIs)
poses an increased risk to global public health and underlines the need to prioritise …

Metagenomic sequencing of municipal wastewater provides a near-complete SARS-CoV-2 genome sequence identified as the B. 1.1. 7 variant of concern from a …

C Landgraff, LYR Wang, C Buchanan, M Wells… - MedRxiv, 2021 - medrxiv.org
Laboratory-based wastewater surveillance for SARS-CoV-2, the causative agent of the
ongoing COVID-19 pandemic, can be conducted using RT-qPCR-based screening of …

Prevalence of SARS-CoV-2 variants in Prague wastewater determined by nanopore-based sequencing

A Dostálková, K Zdeňková, J Bartáčková, E Čermáková… - Chemosphere, 2024 - Elsevier
The early detection of upcoming disease outbreaks is essential to avoid both health and
economic damage. The last four years of COVID-19 pandemic have proven wastewater …

Prevalence and circulation patterns of SARS-CoV-2 variants in European sewage mirror clinical data of 54 European cities

S Agrawal, L Orschler, S Schubert, K Zachmann… - Water research, 2022 - Elsevier
For community-level monitoring, the European Commission under the EU Sewage Sentinel
System recommends wastewater-based SARS-CoV-2 surveillance. Tracking SARS-CoV-2 …

Optimised protocol for monitoring SARS-CoV-2 in wastewater using reverse complement PCR-based whole-genome sequencing

HT Child, PA O'Neill, K Moore, W Rowe, H Denise… - PLoS …, 2023 - journals.plos.org
Monitoring the spread of viral pathogens in the population during epidemics is crucial for
mounting an effective public health response. Understanding the viral lineages that …

Wastewater surveillance of SARS-CoV-2 genomic populations on a country-wide scale through targeted sequencing

F Cancela, N Ramos, DS Smyth, C Etchebehere… - Plos one, 2023 - journals.plos.org
SARS-CoV-2 surveillance of viral populations in wastewater samples is recognized as a
useful tool for monitoring epidemic waves and boosting health preparedness. Next …

SARS-CoV-2 genomic surveillance in wastewater as a model for monitoring evolution of endemic viruses

M Yousif, S Rachida, S Taukobong, N Ndlovu… - Nature …, 2023 - nature.com
As global SARS-CoV-2 burden and testing frequency have decreased, wastewater
surveillance has emerged as a key tool to support clinical surveillance efforts. The aims of …