Identification of human cytomegalovirus isolates by the polymerase chain reaction.

D Zipeto, E Silini, M Parea, E Percivalle… - Microbiologica, 1990 - europepmc.org
Fifty human cytomegalovirus (HCMV) isolates were recovered from different clinical
specimens (buffy coat, throat washing and urine) obtained from fifty patients (23 AIDS …

Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction

K Hsia, DH Spector, J Lawrie… - Journal of Clinical …, 1989 - Am Soc Microbiol
Polymerase chain reaction (PCR) amplification was used to detect human cytomegalovirus
(HCMV) sequences. The fragments selected for amplification were fragments of 130 and …

Polymerase chain reaction assay for detection of human cytomegalovirus

DM Olive, M Simsek, S Al-Mufti - Journal of Clinical Microbiology, 1989 - Am Soc Microbiol
Direct detection of human cytomegalovirus (HCMV) from clinical specimens was examined
by using the polymerase chain reaction (PCR) for amplifying HCMV DNA. The efficiency of …

Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA

RD Allen, PE Pellett, JA Stewart… - Journal of clinical …, 1995 - Am Soc Microbiol
We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus
(HCMV). The assay includes primer and probe sequences derived from conserved HCMV …

Detection of human cytomegalovirus DNA: how, when and where?

G Gerna, F Baldanti, D Zella… - Scandinavian Journal of …, 1995 - europepmc.org
Although several methods have been utilized for the detection and quantification of human
cytomegalovirus (HCMV) DNA, all of them can be divided into three groups:(i) detection of …

Quantification of human cytomegalovirus DNA using the polymerase chain reaction

JC Fox, PD Griffiths, VC Emery - Journal of General …, 1992 - microbiologyresearch.org
The important goal of developing quantitative assays for viral nucleic acids in clinical
samples has been achieved for human cytomegalovirus (HCMV) by using a modified …

Detection of human cytomegalovirus by polymerase chain reaction

MF Mangano, RL Hodinka, JG Spivack - Diagnosis of human viruses by …, 1992 - Springer
Human cytomegalovirus (HCMV) can produce life-threatening disease in
immunosuppressed AIDS patients and organ transplant recipients. Congenital HCMV is the …

Polymerase chain reaction detection of human cytomegalovirus in over 2000 blood specimens correlated with virus isolation and related to urinary virus excretion

E Drouet, S Michelson, G Denoyel… - Journal of virological …, 1993 - Elsevier
The polymerase chain reaction (PCR) as applied to human cytomegalovirus (HCMV)
detection should provide a valuable tool for rapid, reliable diagnosis of infection, thereby …

Diagnosis of cytomegalovirus infections using polymerase chain reaction, virus isolation and serology

W Xu, VA Sundqvist, M Brytting… - Scandinavian journal of …, 1993 - Taylor & Francis
The nested Polymerase Chaain Reaction (PCR) was compared with virus isolation and
serology to establish which is the best method for the diagnosis of active cytomegalovirus …

The use of ELISA and nonradioactive DNA hybridization assays for the detection of human cytomegalovirus

DM Olive, A El Mekki, W Al Mulla, A Kobryn… - Journal of virological …, 1988 - Elsevier
A hybridization assay using a biotinylated DNA probe was compared to both ELISA and
direct isolation methods for detecting human cytomegalovirus (HCMV). The biotin labeled …