Human cytomegalovirus infection: diagnosis by antigen and DNA detection

MG Revello, M Furione, M Zavattoni… - Reviews and Research …, 1994 - journals.lww.com
In recent years there have been major advances in the field of rapid diagnosis of human
cytomegalovirus (HCMV) infections. p72-Specific monoclonal antibodies (mAbs) have led to …

Human cytomegalovirus infection during childhood: detection of viral DNA in peripheral blood by means of polymerase chain reaction

M Shibata, T Morishima, M Terashima… - Medical microbiology …, 1990 - Springer
Polymerase chain reaction (PCR) technique was applied to detect cytomegalovirus (CMV)
DNA. Two pairs of synthetic oligonucleotide primers were used to amplify DNA from the …

Evaluation and comparison of PCR and hybridization methods for rapid detection of cytomegalovirus in clinical samples

R Lévy, F Najioullah, D Thouvenot, S Bosshard… - Journal of virological …, 1996 - Elsevier
Rapid diagnosis of cytomegalovirus (CMV) infection may be obtained by molecular
techniques, such as the polymerase chain reaction (PCR) and hybridization assays. The …

The use of DNA probes in studies of human cytomegalovirus.

SA Spector, DH Spector - Clinical chemistry, 1985 - academic.oup.com
Hybridization assays provide a sensitive and rapid means for studying the molecular biology
of viral replication and for identifying viral nucleic acid in biological specimens. Such assays …

Diagnosis of human cytomegalovirus disease

B Yen-Lieberman - Clinical Microbiology Newsletter, 2000 - Elsevier
Standardization of the various assays used for diagnosis of HCMV disease in different
laboratories is a necessary step before antiviral response to specific therapy can be …

Automated extraction and quantification of human cytomegalovirus DNA in whole blood by real-time PCR assay

C Mengelle, K Sandres-Saune… - Journal of clinical …, 2003 - Am Soc Microbiol
The measurement of human cytomegalovirus (HCMV) DNA in blood is becoming the
standard method for monitoring HCMV infection in immune-suppressed and unsuppressed …

Detection of human cytomegalovirus in urine samples by cell culture, early antigen assay and polymerase chain reaction

A Daiminger, G Schalasta, D Betzl, G Enders - Infection, 1994 - Springer
With the advent of effective therapy rapid, sensitive and reliable assays for diagnosis of
human cytomegalovirus (HCMV) infections are required. In a total of 1,928 urine samples …

Quantitative PCR determination of human cytomegalovirus in blood cells

J Satou, T Funato, N Satoh, Y Abe… - Journal of Clinical …, 2001 - Wiley Online Library
We evaluated a rapid and sensitive method to determine human cytomegalovirus (CMV)
DNA levels in blood cells using a quantitative polymerase chain reaction (PCR) technique …

[HTML][HTML] Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR

S Zhang, YH Zhou, L Li, Y Hu - Virology Journal, 2010 - Springer
Background Human cytomegalovirus (HCMV) infection poses a significant health threat to
immunocompromised individuals. Here we performed this study to set up a highly sensitive …

HCMV‐DNA is detected more frequently than infectious virus in blood leucocytes of immunocompromised patients: A direct comparison of culture …

VM Ratnamohan, JM Mathys… - Journal of medical …, 1992 - Wiley Online Library
In two studies comparing detection of human cytomegalovirus (HCMV) in 118 patients (93 of
whom were immunocompromised) by the polymerase chain reaction (PCR) and virus …