Hairy Root Cultures of Taxus cuspidate for Enhanced Production of Paclitaxel

JA Kim, KH Baek, YM Son, SH Son, H Shin - Journal of the Korean Society …, 2009 - Springer
Hairy root cultures were established by transforming seedlings of Taxus cuspidate Sieb. Et
Zucc (Korean yew) using Agrobacterium rhizogenes. Initially, 107 lines of hairy roots were …

Hairy root cultures of Taxus× media var. Hicksii Rehd. as a new source of paclitaxel and 10-deacetylbaccatin III

M Furmanowa, K Syklowska-Baranek - Biotechnology letters, 2000 - Springer
Hairy root cultures of Taxus× media var. Hicksii were established by infection of the plantlets
with the Agrobacterium rhizogenes strain LBA 9402. The paclitaxel accumulation in hairy …

Hairy Root Induction of Taxus baccata L. by Natural Transformation with Rhizobium rhizogenes

J He, JPA Plácido, I Pateraki, S Kampranis, BT Favero… - Horticulturae, 2022 - mdpi.com
Paclitaxel (Taxol®) is a potent anticancer agent, but the widespread pharmaceutical use of
paclitaxel is hampered by its limited availability due to low accumulation levels in the native …

Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis

HU Yi-Ming, GAN Fan-Yuan, LU Chun-Hua… - Journal of Integrative …, 2003 - jipb.net
A high taxol yield cell line of Taxus yunnanensis Cheng et LK Fu keeps a high taxol-
producing level after successive subcultures for more than eight years. In this study, eight …

Improved paclitaxel production by in situ extraction and elicitation in cell suspension cultures of Taxus chinensis

CH Zhang, HB Xu - Biotechnology letters, 2001 - Springer
Dibutyl phthalate, oleic acid and terpineol were used to extract paclitaxel in situ from Taxus
chinensis suspension cultures. Oleic acid/terpineol (1: 1, v/v) added to the cultures gave a …

Enhancement of taxane production in hairy root culture of Taxus x media var. Hicksii

K Syklowska-Baranek, A Pietrosiuk, A Kokoszka… - Journal of plant …, 2009 - Elsevier
This study assessed the effect of two precursors (l-phenylalanine and p-amino benzoic acid)
used alone or in combination with methyl jasmonate, on the growth and accumulation of …

Protoplast culture and paclitaxel production by Taxus yunnanensis

JP Luo, Q Mu, YH Gu - Plant cell, tissue and organ culture, 1999 - Springer
Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus.
Protoplast yield was dependent on callus age, with a maximum from 20-day-old callus …

Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis

CH Zhang, XG Mei, L Liu, LJ Yu - Biotechnology Letters, 2000 - Springer
Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium.
Addition of 50 mg chitosan l− 1, 60 μM methyl jasmonate and 30 μM Ag+ resulted in the …

Enhancement of Taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal

C Wang, J Wu, X Mei - Applied microbiology and biotechnology, 2001 - Springer
An endophytic fungus, Aspergillus niger, isolated from the inner bark of a Taxus chinensis
tree, was used as an elicitor to stimulate the Taxol (paclitaxel) production in a Taxus …

Paclitaxel production using co-culture of Taxus suspension cells and paclitaxel-producing endophytic fungi in a co-bioreactor

YC Li, WY Tao, L Cheng - Applied microbiology and biotechnology, 2009 - Springer
The co-culture of the suspension cells of Taxus chinensis var. mairei and its endophytic
fungi, Fusarium mairei, in a 20-L co-bioreactor was successfully established for paclitaxel …