Background Bacterial surface display is of interest in many applications, including live
vaccine development, screening of protein libraries and the development of whole cell …

Autotransporters constitute a large family of natural proteins that are essential for delivering
many types of proteins and peptides across the outer membrane in Gram-negative bacteria …

Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

To display a protein or peptide with a distinct function at the surface of a living bacterial cell
is a challenging exercise with constantly increasing impact in many areas of biochemistry …

Highlights•Autotransporters have predominantly been used for surface display on E.
coli.•For industrial applications, host bacteria with specific features are needed.•Here we …

Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …

Background Autotransporters represent a widespread family of secreted proteins in Gram-
negative bacteria. Their seemingly easy secretion mechanism and modular structure make …

Background Bacterial surface display technique enables the exogenous proteins or
polypeptides displayed on the bacterial surface, while maintaining their relatively …