[HTML][HTML] Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuantĘr) system

MM Ewing, JM Thompson, RS McLaren… - Forensic Science …, 2016 - Elsevier
Quantification of the total amount of human DNA isolated from a forensic evidence item is
crucial for DNA normalization prior to short tandem repeat (STR) DNA analysis and a federal …

Development and validation of InnoQuant® HY, a system for quantitation and quality assessment of total human and male DNA using high copy targets

A Loftus, G Murphy, H Brown, A Montgomery… - Forensic Science …, 2017 - Elsevier
The development and validation of InnoQuant® HY, a real-time PCR system containing four
DNA targets—two RE autosomal targets of different sizes, male specific targets, and an …

Production and certification of NIST standard reference material 2372 human DNA quantitation standard

MC Kline, DL Duewer, JC Travis, MV Smith… - Analytical and …, 2009 - Springer
Modern highly multiplexed short tandem repeat (STR) assays used by the forensic human-
identity community require tight control of the initial amount of sample DNA amplified in the …

Development and validation of the Investigator® Quantiplex Pro Kit for qPCR-based examination of the quantity and quality of human DNA in forensic samples

M Vraneš, M Scherer, K Elliott - Forensic Science International: Genetics …, 2017 - Elsevier
We developed and validated the Investigator Quantiplex Pro Kit which quantifies human and
male DNA in a sample using quantitative real-time PCR. An internal PCR control (IPC) is …

A performance study on three qPCR quantification kits and their compatibilities with the 6-dye DNA profiling systems

S Lin, C Li, SCY Ip - Forensic Science International: Genetics, 2018 - Elsevier
DNA quantification plays an integral role in forensic DNA profiling. Not only does it estimate
the total amount of amplifiable human autosomal and male DNA to ensure optimal …

Developmental validation of the Quantifiler® HP and Trio Kits for human DNA quantification in forensic samples

A Holt, SC Wootton, JJ Mulero, PM Brzoska… - Forensic Science …, 2016 - Elsevier
The quantification of human genomic DNA is a necessary first step in the DNA casework
sample analysis workflow. DNA quantification determines optimal sample input amounts for …

[PDF][PDF] Results from the NIST 2004 DNA quantitation study

MC Kline, JW Redman, JM Butler… - Journal of forensic …, 2005 - archive.gfjc.fiu.edu
For optimal DNA short tandem repeat (STR) typing results, the DNA concentration ([DNA]) of
the sample must be accurately determined prior to the polymerase chain reaction (PCR) …

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of …

M Barbisin, R Fang, CE O'Shea… - Journal of forensic …, 2009 - Wiley Online Library
The Quantifiler® Duo DNA Quantification kit enables simultaneous quantification of human
DNA and human male DNA as well as detection of inhibitors of PCR in a single real‐time …

Simultaneous determination of total human and male DNA using a duplex real‐time PCR assay

JA Nicklas, E Buel - Journal of forensic sciences, 2006 - Wiley Online Library
A single duplex assay to determine both the amount of total human DNA and the amount of
male DNA in a forensic sample has been developed. This assay is based on TaqMan™ …

Developmental validation of a real-time PCR assay for the simultaneous quantification of total human and male DNA

BE Krenke, N Nassif, CJ Sprecher, C Knox… - Forensic Science …, 2008 - Elsevier
Multiplex human short tandem repeat analysis demands reliable DNA quantification to
consistently produce interpretable genotypes. The Plexor® HY System is a multiplex …