Effect of acidosis, oxidative stress, and glutamate toxicity on the survival of mature and immature cultured cerebellar granule cells

EV Stelmashuk, EA Belyaeva, NK Isaev - Neurochemical Journal, 2007 - Springer
The effects of various pathogenic factors on the viability of cultured immature and mature
granule cells of rat cerebellum that developed during brain hypoxia and reoxygenation were …

Removal of serum from primary cultures of cerebellar granule neurons induces oxidative stress and DNA fragmentation: protection with antioxidants and glutamate …

C Atabay, CM Cagnoli, E Kharlamov… - Journal of …, 1996 - Wiley Online Library
Cerebellar granule neurons undergo apoptosis when deprived of chronic depolarization;
serum deprivation has not been considered as a trigger of apoptosis in this culture. Here we …

Role of oxidative stress in the apoptotic cell death of cultured cerebellar granule neurons

A Valencia, J Morán - Journal of neuroscience research, 2001 - Wiley Online Library
When cultured cerebellar granule neurons (CGN) are transferred from 25 mM KCl (K25) to 5
mM KCl (K5) caspase‐3 and caspase‐8, but not caspase‐1 or caspase‐9, activities are …

Role of ionic fluxes in the apoptotic cell death of cultured cerebellar granule neurons

A Franco-Cea, A Valencia, S Sánchez-Armass… - Neurochemical …, 2004 - Springer
Cultured cerebellar granule neurons (CGC) increase survival in a medium containing 25
mM KCl (K25), and they die apoptotically when cultures are treated with staurosporine (St) …

Neuronal cell death and reactive oxygen species

A Boldyrev, R Song, VA Dyatlov, DA Lawrence… - Cellular and Molecular …, 2000 - Springer
We have investigated the role of reactive oxygen species (ROS) in cell death induced by
ischemia or application of the excitatory amino acid agonist, N-methyl-D-aspartate (NMDA) …

Characterization of a chemical anoxia model in cerebellar granule neurons using sodium azide: Protection by nifedipine and MK‐801

T Varming, J Drejer, A Frandsen… - Journal of …, 1996 - Wiley Online Library
Induction of chemical anoxia, using sodium azide in cerebellar granule cells maintained in
primary culture, was evaluated as an in vitro assay for screening of potential neuroprotective …

Paradoxical potentiation by low extracellular Ca2+ of acute chemical anoxic neuronal injury in cerebellar granule cell culture.

MA Verity, M Torres, T Sarafian - Molecular and chemical …, 1991 - europepmc.org
Acute chemical anoxic injury was produced in primary cerebellar granule cell cultures
incubated with iodoacetate (IAA) alone or IAA combined with potassium cyanide (KCN) …

Bifonazole modulates death of cultured cerebellar granular cells induced by glutamate and oxygen-glucose deprivation

EV Stel'mashuk, NA Andreeva, L Manukhova… - Bulletin of Experimental …, 2001 - Springer
Abstract Treatment of cultured rat cerebellar granular cells with calmodulin antagonist
bifonazole (10 mM) during oxygen-glucose deprivation or exposure to glutamate (75 mM) …

Early ultrastructural changes after brief histotoxic hypoxia in cultured cortical and hippocampal CA1 neurons

E Dux, U Oschlies, A Uto, M Kusumoto… - Acta …, 1996 - Springer
Primary cortical and hippocampal neuronal cultures submitted to brief histotoxic hypoxia
suffer delayed neuronal death after 24 h [Uto et al.(1995) J Neurochem 64: 2185–2192]. In …

Differential response of immature and mature neurons to hypoxia in rat mesencephalic cultures

N Andreeva, J Heldt, N Leclere, J Gross - Developmental neuroscience, 2002 - karger.com
The effect of hypoxia on immature and mature mesencephalic neurons was studied in in
vitro rat cerebral cell cultures on different days. In immature cultures (6–8 days in vitro) …