The cleavage, metabolism and viability of mouse zygotes were assessed after culture in
media of different ionic and metabolite composition. Medium with a high potassium …

The minimal nutrient requirements of one-cell rabbit embryos for cleavage during in vitro
culture were investigated. One-cell rabbit embryos were cultivated in a simple salt solution …

Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …

The first culture media designed specifically to support development of the preimplantation
mouse embryo were formulated over 50 years ago and were based on balanced salt …

STUDY QUESTION What is the relationship between cleavage stage embryo kinetics,
blastocyst metabolism and subsequent embryo viability? SUMMARY ANSWER Embryos …

An ultramicrofluorometric technique was used to analyse the nutrient composition of mouse
oviduct fluid. The concentrations of pyruvate, glucose and lactate in the vicinity of the …

Culture of in-vivo-developed mouse blastocysts in a simple culture medium based on a
balanced salt solution supplemented with carbohydrates for 3 h significantly perturbed …

A limiting factor in the development of new technologies and transport of rats worldwide has
been the inability to robustly culture preimplantation embryos. Previously, culture in vitro to …

Since the inception of modern embryo culture media over 50 years ago there have been
significant developments in culture systems for the mammalian preimplantation embryo …

Objective To determine compositions of commercial single-step culture media and test with
a murine model whether differences in composition are biologically relevant. Design …