A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications

A Haque, J Engel, SA Teichmann, T Lönnberg - Genome medicine, 2017 - Springer
RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative
analysis of messenger RNA molecules in a biological sample and is useful for studying …

Introduction to single‐cell RNA sequencing

TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …

A multicenter study benchmarking single-cell RNA sequencing technologies using reference samples

W Chen, Y Zhao, X Chen, Z Yang, X Xu, Y Bi… - Nature …, 2021 - nature.com
Comparing diverse single-cell RNA sequencing (scRNA-seq) datasets generated by
different technologies and in different laboratories remains a major challenge. Here we …

Single-cell RNA-seq technologies and related computational data analysis

G Chen, B Ning, T Shi - Frontiers in genetics, 2019 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …

Single-cell RNA sequencing analysis: a step-by-step overview

S Slovin, A Carissimo, F Panariello, A Grimaldi… - RNA bioinformatics, 2021 - Springer
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications

M Su, T Pan, QZ Chen, WW Zhou, Y Gong, G Xu… - Military Medical …, 2022 - Springer
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has
advanced our understanding of the pathogenesis of disease and provided valuable insights …

Design and computational analysis of single-cell RNA-sequencing experiments

R Bacher, C Kendziorski - Genome biology, 2016 - Springer
Single-cell RNA-sequencing (scRNA-seq) has emerged as a revolutionary tool that allows
us to address scientific questions that eluded examination just a few years ago. With the …

Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Single-cell RNA sequencing and its combination with protein and DNA analyses

JR Choi, KW Yong, JY Choi, AC Cowie - Cells, 2020 - mdpi.com
Heterogeneity in cell populations poses a significant challenge for understanding complex
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …

[HTML][HTML] Comparative analysis of single-cell RNA sequencing methods

C Ziegenhain, B Vieth, S Parekh, B Reinius… - Molecular cell, 2017 - cell.com
Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and
medical questions. However, systematic comparisons of the performance of diverse scRNA …