The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level
production in bacterial hosts and simple optimisation protocols have not been available yet …

Bacterial inclusion bodies (IBs) are refractile aggregates of protease-resistant misfolded
protein that often occur in recombinant bacteria upon gratuitous overexpression of cloned …

High-level expression of recombinant proteins in Escherichia coli often results in
accumulation of protein molecules into aggregates known as inclusion bodies (IBs) …

The vast majority of protein purification is now done with cloned, recombinant proteins
expressed in a suitable host. The predominant host is Escherichia coli. Many, if not most …

Recent advances in generating active proteins through refolding of bacterial inclusion body
proteins are summarized in conjunction with a short overview on inclusion body isolation …

Abstract Background Bacterial inclusion bodies (IBs) are key intermediates for protein
production. Their quality affects the refolding yield and further purification. Recent functional …

The bacterium Escherichia coli is a major host for recombinant protein production of non-
glycosylated products. Depending on the expression strategy, the recombinant protein can …

Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

Human G‐CSF (granulocyte colony‐stimulating factor) is a well‐known biopharmaceutical
drug being mostly produced by overexpression in Escherichia coli, where it appears in the …

Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving
tremendous attention for many diverse applications in the areas of industrial and medical …