Development of a sandwich ELISA to detect IgG and IgG sub‐class antibodies specific for a major antigen (Ag 7) of Aspergillus fumigatus

C Harvey, JL Longbottom - Clinical & Experimental Allergy, 1986 - Wiley Online Library
A sandwich ELISA has been developed, using an affinity purified monospecific antiserum as
a capture antibody, to detect specific IgG and IgG sub‐classes to a major antigen (Ag 7) of …

Class-Specific Antibody Determination against Aspergillus fumigatus by Means of the Enzyme-Linked Immunosorbent Assay: III. Comparative Study: IgG, IgA, IgM …

HF Kauffman, S Van der Heide, F Beaumont… - … Archives of Allergy and …, 1986 - karger.com
Abstract IgG, IgA and IgM ELISA antibody titers against Aspergillus fumigatus were elevated
in sera of patients with aspergilloma and allergic bronchopulmonary aspergillosis (ABPA) …

Antibodies against Aspergillus fumigatus II. Identification and Quantification by Means of Crossed Immunoelectrophoresis

HF Kauffmann, K De Vries - International Archives of Allergy and …, 1980 - karger.com
Individual antigenic components of Aspergillus fumigatus were identified by crossed
immunoelectrophoresis (CIE) using pooled rabbit hyperimmune antiserum. Patients' sera …

Characterization of Immunologically Important Antigens and Allergens of Aspergillus fumigatus

JL Longbottom, C Harvey, ML Taylor… - … Archives of Allergy and …, 1989 - karger.com
Using a variety of immunochemical methods, including quantitative immuno-electrophoretic
techniques, combined with gel filtration and iso-electric focusing, and production of …

Isolation and Characterization of a Relevant Aspergillus fumigatus Antigen with IgG- and IgE-Binding Activity

VP Kurup, M Ramasamy, PA Greenberger… - International Archives of …, 1988 - karger.com
An immunologically relevant antigen fraction was isolated from a cytoplasmic extract of
Aspergillus fumigatus mycelium. The fraction was obtained by concanavalin-A affinity …

Antigen and antibody attachment in ELISA for Aspergillus fumigatus IgG antibodies

MD Richardson, DW Warnock - Journal of immunological methods, 1984 - Elsevier
Four commercial antigen extracts of Aspergillus fumigatus were evaluated for use in a rapid
enzyme-linked immunosorbent assay (ELISA) for anti-A. fumigatus IgG. Initial binding of both …

Studies of the dual antibody response in allergic bronchopulmonary aspergillosis

GB Goldstein, M Yokoyama - Journal of Allergy, 1970 - Elsevier
Bronchopulmonary aspergillosis was diagnosed in a 22-year-old man with pulmonary
infiltrates and peripheral eosinophilia on the basis of precipitating antibody to A. fumigatus in …

Antibody isotype responses in Aspergillus-induced diseases

VP Kurup, A Resnick, J Kalbfleish… - The Journal of …, 1990 - translationalres.com
The Aspergillus fumigatus-specific immunoglobulin class and subclasses were evaluated in
the sera of patients with allergic bronchopulmonary asperglliosis, aspergllioma, patients with …

Optimized preparation of Aspergillus fumigatus extracts for allergy diagnosis

SA Little, JL Longbottom… - Clinical & Experimental …, 1993 - Wiley Online Library
Extracts of Aspergillus fumigatus are required for the measurement of specific antibodies
that are important indices in the diagnosis of allergic bronchopulmonary aspergillosis …

Modified indirect fluorescent-antibody test for aspergillosis

MA Gordon, EW Lapa, J Kane - Journal of Clinical Microbiology, 1977 - Am Soc Microbiol
Diffuse fluorescence in an indirect fluorescent-antibody test for antibody to Aspergillus
fumigatus was eliminated by using germlings, rather than hyphae, as antigen. The modified …