As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …

Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …

A variety of sequence-specific nucleases (SSNs) have been successfully used for plant
genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR) …

The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome
editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free …

The complex allotetraploid genome is one of major challenges in cotton for repressing gene
expression. Developing site-specific DNA mutation is the long-term dream for cotton …

The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …

Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …

Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …

Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for
genome editing. A new effector with a single nuclease domain, a relatively small size, low …

Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …