[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)

B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …

High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system

P Wang, J Zhang, L Sun, Y Ma, J Xu… - Plant biotechnology …, 2018 - Wiley Online Library
Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …

[HTML][HTML] Enhanced genome editing in rice using single transcript unit CRISPR‐LbCpf1 systems

R Xu, R Qin, H Li, J Li, J Yang, P Wei - Plant biotechnology journal, 2019 - ncbi.nlm.nih.gov
A variety of sequence-specific nucleases (SSNs) have been successfully used for plant
genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR) …

[HTML][HTML] The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of …

B Li, S Liang, M Alariqi, F Wang, G Wang… - Plant biotechnology …, 2021 - ncbi.nlm.nih.gov
The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome
editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free …

[HTML][HTML] A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)

C Li, T Unver, B Zhang - Scientific reports, 2017 - nature.com
The complex allotetraploid genome is one of major challenges in cotton for repressing gene
expression. Developing site-specific DNA mutation is the long-term dream for cotton …

[PDF][PDF] Expanding the scope of CRISPR/Cpf1-mediated genome editing in rice

S Li, X Zhang, W Wang, X Guo, Z Wu, W Du, Y Zhao… - Molecular Plant, 2018 - cell.com
The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …

[HTML][HTML] Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

X Chen, X Lu, N Shu, S Wang, J Wang, D Wang… - Scientific reports, 2017 - nature.com
Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …

[HTML][HTML] Genome editing in cotton with the CRISPR/Cas9 system

W Gao, L Long, X Tian, F Xu, J Liu, PK Singh… - Frontiers in plant …, 2017 - frontiersin.org
Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …

The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G. hirsutum) plants

Q Wang, M Alariqi, F Wang, B Li, X Ding… - Plant biotechnology …, 2020 - Wiley Online Library
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for
genome editing. A new effector with a single nuclease domain, a relatively small size, low …

[HTML][HTML] Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

L Long, DD Guo, W Gao, WW Yang, LP Hou, XN Ma… - Plant Methods, 2018 - Springer
Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …