[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)
B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …
High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system
P Wang, J Zhang, L Sun, Y Ma, J Xu… - Plant biotechnology …, 2018 - Wiley Online Library
Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …
[HTML][HTML] Enhanced genome editing in rice using single transcript unit CRISPR‐LbCpf1 systems
A variety of sequence-specific nucleases (SSNs) have been successfully used for plant
genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR) …
genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR) …
[HTML][HTML] The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of …
The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome
editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free …
editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free …
A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)
The complex allotetraploid genome is one of major challenges in cotton for repressing gene
expression. Developing site-specific DNA mutation is the long-term dream for cotton …
expression. Developing site-specific DNA mutation is the long-term dream for cotton …
[PDF][PDF] Expanding the scope of CRISPR/Cpf1-mediated genome editing in rice
The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system
X Chen, X Lu, N Shu, S Wang, J Wang, D Wang… - Scientific reports, 2017 - nature.com
Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …
system has been widely used for genome editing in various plants because of its simplicity …
[HTML][HTML] Genome editing in cotton with the CRISPR/Cas9 system
Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …
The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G. hirsutum) plants
Q Wang, M Alariqi, F Wang, B Li, X Ding… - Plant biotechnology …, 2020 - Wiley Online Library
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for
genome editing. A new effector with a single nuclease domain, a relatively small size, low …
genome editing. A new effector with a single nuclease domain, a relatively small size, low …
Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
L Long, DD Guo, W Gao, WW Yang, LP Hou, XN Ma… - Plant Methods, 2018 - Springer
Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …