The current application of genome editing to crop plants is limited to cultivars that are
amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing …

Genome editing can be used to create new wheat varieties with enhanced performance.
Clustered regularly interspaced short palindromic repeat (CRISPR) is a powerful tool for …

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

Plant virus-mediated sgRNA delivery and expression have great advantages; sgRNA
expression can rapidly expand and accumulate along with virus replication and movement …

CRISPR/Cas9 has been widely used for genome editing in many organisms, including
important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the …

The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …

Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the
world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR …

Gossypium hirsutum is an allotetraploid species, meaning that mutants that are difficult to be
generated by classical approaches due to gene redundancy. The CRISPR/Cas9 genome …

Many Cas9-derived base editors have been developed for precise C–to–T and A–to–G base
editing in plants (Molla et al., 2021). They are typically based on a SpCas9 nickase or its …

CRISPR-Cas9,-Cas12a,-Cas12b, and-Cas13 have been harnessed for genome
engineering in human and plant cells (Liu et al., 2022). However, the large size of these Cas …