In recent decades, Pakistan has suffered a decline in cotton production due to several
factors, including insect pests, cotton leaf curl disease (CLCuD), and multiple abiotic …

Common wheat (Triticum aestivum) is one of the most widely cultivated and consumed crops
globally. In the face of limited arable land and climate changes, it is a great challenge to …

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …

Common wheat supplies vast amounts of dietary carbohydrate and protein for over 60% of
world population. With continued economic development, demand for high-yielding varieties …

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-
associated Cas9) has been widely used in genome editing in a variety of organisms …

Plant breeding is one of the oldest sustainable agriculture methods used to increase the
yield, quality and other biomaterial for human use. Many crops like fruits, vegetables …

The Streptococcus pyogenes CRISPR-Cas9 system effectively mediates RNA-guided DNA
double-strand breaks and is used for genome editing in many different organisms, including …

This protocol is an extension to: Nat. Protoc. 9, 2395–2410 (2014); doi: 10.1038/nprot.
2014.157; published online 18 September 2014 In recent years, CRISPR/Cas9 has …

Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …

Background CRISPR/Cas9-based genome editing holds great promise to accelerate the
development of improved crop varieties by providing a powerful tool to modify the genomic …