Highly efficient genome editing in plant protoplasts by ribonucleoprotein delivery of CRISPR-Cas12a nucleases
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome
editing is a powerful approach for crop improvement. Traditional transformation methods …
editing is a powerful approach for crop improvement. Traditional transformation methods …
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop
breeding. The avoidance of transgene integration and reduction of off-target mutations are …
breeding. The avoidance of transgene integration and reduction of off-target mutations are …
Plant genome editing using FnCpf1 and LbCpf1 nucleases at redefined and altered PAM sites
CRISPR from Prevotella and Francisella 1 (Cpf1) is an emerging RNA-guided
endonuclease system that relies on thymidine-rich protospacer adjacent motif (PAM) for …
endonuclease system that relies on thymidine-rich protospacer adjacent motif (PAM) for …
Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat
S Zhang, R Zhang, G Song, J Gao, W Li, X Han… - BMC plant biology, 2018 - Springer
Abstract Background Recently, the CRISPR/Cas9 system has been widely used to precisely
edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation …
edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation …
Challenges facing CRISPR/Cas9-based genome editing in plants
S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …
security. The revolution of genome editing technologies based on the clustered regularly …
Engineering herbicide-resistant rice plants through CRISPR/Cas9-mediated homologous recombination of acetolactate synthase
Genome editing technologies enable precise modifications of DNA sequences in vivo and
offer great promise for crop improvement. CRISPR/Cas9 (Clustered Regularly Interspaced …
offer great promise for crop improvement. CRISPR/Cas9 (Clustered Regularly Interspaced …
A modular cloning toolkit for genome editing in plants
Background CRISPR/Cas has recently become a widely used genome editing tool in
various organisms, including plants. Applying CRISPR/Cas often requires delivering …
various organisms, including plants. Applying CRISPR/Cas often requires delivering …
CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum
J Gao, G Wang, S Ma, X Xie, X Wu, X Zhang, Y Wu… - Plant molecular …, 2015 - Springer
Genome editing is one of the most powerful tools for revealing gene function and improving
crop plants. Recently, RNA-guided genome editing using the type II clustered regularly …
crop plants. Recently, RNA-guided genome editing using the type II clustered regularly …
The ScCas9++ variant expands the CRISPR toolbox for genome editing in plants
T Liu, D Zeng, Z Zheng, Z Lin, Y Xue… - Journal of integrative …, 2021 - Wiley Online Library
The development of clustered regularly interspaced palindromic repeats (CRISPR)‐
associated protein (Cas) variants with a broader recognition scope is critical for further …
associated protein (Cas) variants with a broader recognition scope is critical for further …
Whole genome sequencing reveals rare off‐target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9‐edited cotton plants
J Li, H Manghwar, L Sun, P Wang… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas9 system has been extensively applied for crop improvement. However,
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …