Abstracts Processes of traditional trait development in plants depend on genetic variations
derived from spontaneous mutation or artificial random mutagenesis. Limited availability of …

Advances in genome editing technologies have tremendous potential to address the
limitations of classical resistance breeding. CRISPR-Cas9 based gene editing has been …

CRISPR/Cas9 is a newly developed and naturally occurred genome editing tool, which is
originally used by bacteria for immune defence. In the past years, it has been quickly …

The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …

Clustered regularly interspaced short palindromic repeats‐associated protein 9 (CRISPR‐
Cas9) is a revolutionary technology that enables efficient genomic modification in many …

Generating mutants bearing multiple gene modifications is essential for 1 determining the
functions of gene family members with redundant functions, or for 2 analyzing epistatic …

Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and
induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA …

Main conclusion An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-
driven Cas9 exhibits consistently high mutation efficiency in Arabidopsis and M. truncatula …

Traditional breeding techniques are proven, but additional knowledge learned from genome
sequencing provides vast new data that might help identify gene targets for improving cotton …

Pea (Pisum sativum L.) is an annual cool-season legume crop. Owing to its role in
sustainable agriculture as both a rotation and a cash crop, its global market is expanding …