CRISPR/Cas system: recent advances and future prospects for genome editing

H Manghwar, K Lindsey, X Zhang, S Jin - Trends in plant science, 2019 - cell.com
Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …

Gene editing by CRISPR/Cas9 in the obligatory outcrossing Medicago sativa

R Gao, BA Feyissa, M Croft, A Hannoufa - Planta, 2018 - Springer
Abstract Main conclusion The CRISPR/Cas9 technique was successfully used to edit the
genome of the obligatory outcrossing plant species Medicago sativa L.(alfalfa). RNA-guided …

Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: Current state and future prospects

DL Singha, D Das, YN Sarki, N Chowdhury, M Sharma… - Planta, 2022 - Springer
Main conclusion In a nutshell, tissue-specific CRISPR/Cas genome editing is the most
promising approach for crop improvement which can bypass the hurdle associated with …

Efficient CRISPR/Cas9-mediated genome editing using a chimeric single-guide RNA molecule

H Butt, A Eid, Z Ali, MAM Atia, MM Mokhtar… - Frontiers in plant …, 2017 - frontiersin.org
The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted
mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous …

Modern trends in plant genome editing: an inclusive review of the CRISPR/Cas9 toolbox

A Razzaq, F Saleem, M Kanwal, G Mustafa… - International journal of …, 2019 - mdpi.com
Increasing agricultural productivity via modern breeding strategies is of prime interest to
attain global food security. An array of biotic and abiotic stressors affect productivity as well …

CRISPR/Cas9-based genome editing in plants

Y Zhang, X Ma, X Xie, YG Liu - Progress in molecular biology and …, 2017 - Elsevier
Recently, genome editing technologies have shown great potential in plants. The newly
developed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system …

Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases

MB Begemann, BN Gray, E January, GC Gordon… - Scientific reports, 2017 - nature.com
Precise genome editing of plants has the potential to reshape global agriculture through the
targeted engineering of endogenous pathways or the introduction of new traits. To develop a …

Generation of targeted point mutations in rice by a modified CRISPR/Cas9 system

J Li, Y Sun, J Du, Y Zhao, L Xia - Molecular plant, 2017 - cell.com
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-
associated Cas9 endonuclease)-mediated genome editing has revolutionized biological …

[HTML][HTML] Multiplex genome editing in Ashbya gossypii using CRISPR-Cpf1

A Jiménez, B Hoff, JL Revuelta - New biotechnology, 2020 - Elsevier
CRISPR/Cas technologies constitute essential tools for rapid genome engineering of many
organisms, including fungi. The CRISPR/Cas9 system adapted for the industrial fungus …

[PDF][PDF] Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice

X Meng, X Hu, Q Liu, X Song, C Gao… - Science China. Life …, 2018 - gaolab.genetics.ac.cn
The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) system
has been widely used for a variety of applications, including targeted gene knockout, gene …