CRISPR/Cas9 systems are nowadays applied extensively to effect genome editing in
various organisms including plants. CRISPR from Prevotella and Francisella 1 (Cpf1) is a …

Abstract Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and
requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered …

Common wheat (Triticum aestivum L.) is one of the three major food crops in the world; thus,
wheat breeding programs are important for world food security. Characterizing the genes …

Recent developments in genome editing techniques have aroused substantial excitement
among agricultural scientists. These techniques offer new opportunities for developing …

CRISPR‐Cas9 and Cas12a are two powerful genome editing systems. Expression of
CRISPR in plants is typically achieved with a mixed dual promoter system, in which Cas …

Targeted genome-editing tools are very important and efficient for plant functional genomics
research, especially when proper mutants are not available. CRISPR/Cas9-based genome …

Genome editing of model organisms is essential for gene function analysis and is thus
critical for human health and agricultural production. The current technologies used for …

Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9),
sourced from a human pathogen, is the most widely used. Here, through in silico data …

Background Targeted genome editing using the Clustered Regularly Interspaced Short
Palindromic Repeats (CRISPR)/Cas9 system has been applied in a large number of plant …