Dual luciferase gene reporter assays to study miRNA function
T Clément, V Salone, M Rederstorff - Small non-coding RNAs: methods …, 2015 - Springer
This chapter describes one of the most reliable quantitative assays to test the silencing of a
possible target gene by a specific miRNA using a luciferase reporter gene. The experimental …
possible target gene by a specific miRNA using a luciferase reporter gene. The experimental …
Experimental validation of microRNA targets using a luciferase reporter system
FE Nicolas - MicroRNAs in Development: Methods and Protocols, 2011 - Springer
MicroRNAs (miRNAs) are a class of small noncoding transcripts that repress gene
expression by pairing with their target messenger RNAs (mRNAs). The human genome …
expression by pairing with their target messenger RNAs (mRNAs). The human genome …
The guideline of the design and validation of MiRNA mimics
Z Wang - MicroRNA and Cancer: Methods and Protocols, 2011 - Springer
The miRNA mimic technology (miR-Mimic) is an innovative approach for gene silencing.
This approach is to generate nonnatural double-stranded miRNA-like RNA fragments. Such …
This approach is to generate nonnatural double-stranded miRNA-like RNA fragments. Such …
Transcriptional gene silencing in mammalian cells by miRNA mimics that target gene promoters
ST Younger, DR Corey - Nucleic acids research, 2011 - academic.oup.com
Synthetic small duplex RNAs that are fully complementary to gene promoters can silence
transcription in mammalian cells. microRNAs (miRNAs) are endogenous small regulatory …
transcription in mammalian cells. microRNAs (miRNAs) are endogenous small regulatory …
Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
RJ Hsu, HJ Yang, HJ Tsai - Nucleic acids research, 2009 - academic.oup.com
We developed a simple, direct and cost-effective approach to search for the most likely
target genes of a known microRNA (miRNA) in vitro. We term this method 'labeled miRNA …
target genes of a known microRNA (miRNA) in vitro. We term this method 'labeled miRNA …
A biochemical approach to identifying microRNA targets
Identifying the downstream targets of microRNAs (miRNAs) is essential to understanding
cellular regulatory networks. We devised a direct biochemical method for miRNA target …
cellular regulatory networks. We devised a direct biochemical method for miRNA target …
Sequence-specific inhibition of microRNA-and siRNA-induced RNA silencing
A large number of miRNAs have recently been discovered in plants and animals.
Development of reverse genetic approaches that act to inhibit microRNA function would …
Development of reverse genetic approaches that act to inhibit microRNA function would …
A novel method to detect functional microRNA targets
S Vatolin, K Navaratne, RJ Weil - Journal of molecular biology, 2006 - Elsevier
MicroRNA (miRNA) molecules are non-coding RNAs, 19 to 24nt in length that have been
identified recently as important regulators of gene expression. Several computational …
identified recently as important regulators of gene expression. Several computational …
Reexamining assumptions about miRNA-guided gene silencing
MicroRNAs (miRNAs) are short endogenously expressed RNAs that have the potential to
regulate the expression of any RNA. This potential has led to the publication of several …
regulate the expression of any RNA. This potential has led to the publication of several …
Identification and analysis of microRNAs
S Bagga, AE Pasquinelli - Genetic Engineering: Principles and Methods, 2006 - Springer
The first microRNA (miRNA) gene was uncovered in 1993. After languishing in near
obscurity for almost a decade, this gene is now recognized as the founding member of a …
obscurity for almost a decade, this gene is now recognized as the founding member of a …