Astrocytes tile the entire CNS. They are vital for neural circuit function, but have traditionally
been viewed as simple, homogenous cells that serve the same essential supportive roles …

Rare cells have an important role in development and disease, and methods for isolating
and studying cell subsets are therefore an essential part of biology research. Such methods …

Astrocytes are a predominant glial cell type in the nervous systems, and are becoming
recognized as important mediators of normal brain function as well as neurodevelopmental …

Brain–immune cross-talk and neuroinflammation critically shape brain physiology in health
and disease. A detailed understanding of the brain immune landscape is essential for …

Cellular diversification is critical for specialized functions of the brain including learning and
memory. Single-cell RNA sequencing facilitates transcriptomic profiling of distinct major …

Multimodal astrocyte–neuron communications govern brain circuitry assembly and function.
For example, through rapid glutamate release, astrocytes can control excitability, plasticity …

Astrocyte heterogeneity is a rapidly evolving field driven by innovative techniques.
Inflammatory astrocytes, one of the first described subtypes of reactive astrocytes, are …

Droplet-based single-cell RNA-seq has emerged as a powerful technique for massively
parallel cellular profiling. While this approach offers the exciting promise to deconvolute …

Microglia are increasingly recognized for their major contributions during brain development
and neurodegenerative disease. It is currently unknown whether these functions are carried …

A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues
into single-cell suspensions. While many protocols have been optimized for optimal cell …