A simple and efficient cryopreservation protocol using encapsulation-dehydration was
established for in vitro-grown shoot-tips of apple 'Gala'(Malus× domestica Borkh.). Shoot …

Shoot tips sampled on in vitro cultured apple plantlets of 6 accessions of M. domestica and
one accession of M. robusta were successfully cryopreserved using the encapsulation …

Axillary shoot tips of apple cv. Golden Delicious isolated from shoot cultures were
successfully cryopreserved using the encapsulation-dehydration technique. After …

Here, we report an efficient and widely applicable method for cryopreservation of Malus
shoot tips by encapsulation–dehydration using adventitious shoots. Shoots were induced …

In this paper, the efficiency of three techniques (two-step freezing, vitrification, encapsulation-
dehydration) for freezing in vitro shoot tips was compared and the effect of conditioning of …

In vitro-grown shoot tips of apples (Malus domestica Borkh. cv. Fuji) were successfully
cryopreserved by vitrification. Three-week-old in vitro apple plantlets were cold-hardened at …

Shoot tips excised from apple (Malus domestica Borkh. cv. Golden Delicious) dormant buds
immediately before freezing were successfully cryopreserved using three different methods …

Alginate-coated shoot tips from in vitro-grown apple (Malus domestica Borkh cv. Fuji) were
successfully cryopreserved following dehydration. Shoot tips cold-hardened at 5° C for 3 …

Shoot tips sampled on in vitro plantlets of 5 apple cultivars were successfully cryopreserved
using the droplet freezing method. After 3 weeks of cold-hardening at 5 C, the shoot tips …

In vitro axillary buds of two apple cultivars, Pinova and Jonagold, were successfully
cryopreserved by droplet-vitrification. In vitro axillary buds of cv. Pinova were subjected to …