Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)
Abstract Background Base editors (BEs) display diverse applications in a variety of plant
species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have …
species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have …
High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system
The base‐editing technique using CRISPR/nC as9 (Cas9 nickase) or dC as9 (deactivated
Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this …
Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this …
Simplified adenine base editors improve adenine base editing efficiency in rice
K Hua, X Tao, W Liang, Z Zhang… - Plant Biotechnology …, 2020 - Wiley Online Library
Adenine base editors (ABE s) have been exploited to introduce targeted adenine (A) to
guanine (G) base conversions in various plant genomes, including rice, wheat and …
guanine (G) base conversions in various plant genomes, including rice, wheat and …
PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants
J Tan, D Zeng, Y Zhao, Y Wang, T Liu… - Plant Biotechnology …, 2022 - Wiley Online Library
Adenine base editors (ABEs), which are generally engineered adenosine deaminases and
Cas variants, introduce site‐specific A‐to‐G mutations for agronomic trait improvement …
Cas variants, introduce site‐specific A‐to‐G mutations for agronomic trait improvement …
ABE8e with Polycistronic tRNA-gRNA Expression Cassette Sig-Nificantly Improves Adenine Base Editing Efficiency in Nicotiana benthamiana
Z Wang, X Liu, X Xie, L Deng, H Zheng, H Pan… - International Journal of …, 2021 - mdpi.com
Adenine base editor containing TadA8e (ABE8e) has been reported in rice. However, the
application of ABE8e in other plant species has not been described, and the comparison …
application of ABE8e in other plant species has not been described, and the comparison …
A large-scale genome and transcriptome sequencing analysis reveals the mutation landscapes induced by high-activity adenine base editors in plants
S Li, L Liu, W Sun, X Zhou, H Zhou - Genome Biology, 2022 - Springer
Background The high-activity adenine base editors (ABEs), engineered with the recently-
developed tRNA adenosine deaminases (TadA8e and TadA9), show robust base editing …
developed tRNA adenosine deaminases (TadA8e and TadA9), show robust base editing …
High-efficiency and multiplex adenine base editing in plants using new TadA variants
D Yan, B Ren, L Liu, F Yan, S Li, G Wang, W Sun… - Molecular plant, 2021 - cell.com
Recently reported adenine base editors (ABEs) exhibit powerful potential for targeted gene
correction as well as developing gain-of-function mutants and novel germplasms for both …
correction as well as developing gain-of-function mutants and novel germplasms for both …
High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system
P Wang, J Zhang, L Sun, Y Ma, J Xu… - Plant biotechnology …, 2018 - Wiley Online Library
Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …
[HTML][HTML] CRISPR/Cas9-mediated adenine base editing in rice genome
LI Hao, QIN Ruiying, LIU Xiaoshuang, L Shengxiang… - Rice Science, 2019 - Elsevier
Precise base editing is highly desired in plant functional genomic research and crop
molecular breeding. In this study, we constructed a rice-codon optimized adenine base …
molecular breeding. In this study, we constructed a rice-codon optimized adenine base …
[HTML][HTML] Generation of a high-efficiency adenine base editor with TadA8e for developing wheat dinitroaniline-resistant germplasm
H Han, Z Wu, L Zheng, J Han, Y Zhang, J Li, S Zhang… - The Crop Journal, 2022 - Elsevier
Base editing using CRISPR technologies is an invaluable tool for crop breeding. One of the
major base editors, the adenine base editor (ABE), has been successfully used in both …
major base editors, the adenine base editor (ABE), has been successfully used in both …