Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

C Li, Y Zong, Y Wang, S Jin, D Zhang, Q Song… - Genome biology, 2018 - Springer
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine.
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …

[PDF][PDF] Precise A· T to G· C base editing in the rice genome

K Hua, X Tao, F Yuan, D Wang, JK Zhu - Molecular plant, 2018 - cell.com
Precise modification of eukaryotic genomes has been accomplished mainly through
homology-directed repair (HDR) of DNA double-strand breaks (DSBs)(Hess et al., 2017) …

[HTML][HTML] Increasing cytosine base editing scope and efficiency with engineered Cas9-PmCDA1 fusions and the modified sgRNA in rice

Y Wu, W Xu, F Wang, S Zhao, F Feng, J Song… - Frontiers in …, 2019 - frontiersin.org
Base editors that do not require double-stranded DNA cleavage or homology-directed repair
enable higher efficiency and cleaner substitution of targeted single nucleotides in genomic …

Development of an efficient plant dual cytosine and adenine editor

R Xu, F Kong, R Qin, J Li, X Liu… - Journal of Integrative …, 2021 - Wiley Online Library
An enhanced CDA‐like (eCDAL) was established from Japanese lamprey CDA1‐like 4 to
achieve a high editing frequency in a broad region as a C‐terminal cytosine base editors …

[PDF][PDF] Improved base editor for efficiently inducing genetic variations in rice with CRISPR/Cas9-guided hyperactive hAID mutant

B Ren, F Yan, Y Kuang, N Li, D Zhang, X Zhou, H Lin… - Molecular plant, 2018 - cell.com
Single-nucleotide polymorphism (SNP) is an abundant form of genetic variation among
individuals in plant species, which has been believed to have effects on the gene function …

The ScCas9++ variant expands the CRISPR toolbox for genome editing in plants

T Liu, D Zeng, Z Zheng, Z Lin, Y Xue… - Journal of integrative …, 2021 - Wiley Online Library
The development of clustered regularly interspaced palindromic repeats (CRISPR)‐
associated protein (Cas) variants with a broader recognition scope is critical for further …

[HTML][HTML] Efficient C‐to‐G editing in rice using an optimized base editor

Y Tian, R Shen, Z Li, Q Yao, X Zhang… - Plant Biotechnology …, 2022 - ncbi.nlm.nih.gov
Cytosine and adenosine base editors (CBE and ABE) have been vigorously developed in
plants, but the base conversion types are limited (Ren et al., 2018, 2021; Xu et al., 2021). A …

[HTML][HTML] Highly efficient C‐to‐T and A‐to‐G base editing in a Populus hybrid

G Li, S Sretenovic, E Eisenstein… - Plant Biotechnology …, 2021 - ncbi.nlm.nih.gov
CRISPR-Cas-based genome editing technologies hold great potential for genetic research
and bioengineering in trees. CRISPR-Cas9 was previously demonstrated to be a highly …

Efficient Genome Editing in Setaria italica Using CRISPR/Cas9 and Base Editors

Z Liang, Y Wu, L Ma, Y Guo, Y Ran - Frontiers in Plant Science, 2022 - frontiersin.org
The genome editing toolbox based on CRISPR/Cas9 has brought revolutionary changes to
agricultural and plant scientific research. With the development of stable genetic …

[HTML][HTML] The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton (G. hirsutum) and creation of …

B Li, S Liang, M Alariqi, F Wang, G Wang… - Plant biotechnology …, 2021 - ncbi.nlm.nih.gov
The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome
editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free …