Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for
genome editing. A new effector with a single nuclease domain, a relatively small size, low …

An adenine base editor with expanded targeting scope using SpCas9‐NGv1 in rice - PMC Back
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Applications of adenine base editors (ABEs) have been constrained by the limited
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …

Base editing tools with diversified editing scopes and minimized RNA off-target activities are
required for broad applications. Nevertheless, current Streptococcus pyogenes Cas9 …

The CRISPR/Cas9 system has been extensively applied for crop improvement. However,
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …

Adenine and cytosine base editors (ABE, CBE) allow for precision genome engineering.
Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced …

accepted 6 November 2020.* Correspondence Leena Tripathi (Tel+ 254-20-422-3472; fax+
254-20-422-3001; L. Tripathi@ cgiar. org), Kan Wang (Tel+ 1-515-294-4429; fax+ 1-515 …

Phytopathogenic bacteria play important roles in plant productivity, and developments in
gene editing have potential for enhancing the genetic tools for the identification of critical …

CRISPR technologies enabling precise genome manipulation are valuable for gene function
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …

Cotton (Gossypium spp.) is one of the most important fiber crops worldwide. In the last two
decades, transgenesis and genome editing have played important roles in cotton …