The biogenesis of connexins and their assembly into functional gap junction hemichannels
(connexons) was studied with the use of a cell-free transcription/translation system. Velocity …

Several different gap junction channel subunit isotypes, known as connexins, were
synthesized in a cell-free translation system supplemented with microsomal membranes to …

Connexins, the proteins that form gap junction channels, are polytopic plasma membrane
(PM) proteins that traverse the plasma membrane bilayer four times. The insertion of five …

Gap-junction channels provide a widespread intercellular signalling mechanism. They are
constructed of a family of connexin membrane proteins that thread across the membrane …

To study the assembly of gap junctions, connexin-green-fluorescent-protein (Cx-GFP)
chimeras were expressed in COS-7 and HeLa cells. Cx26-and Cx32-GFP were targeted to …

Many cells contain two (or more) gap junction proteins that are able to oligomerize with each
other to form heteromeric gap junction channels and influence the properties of intercellular …

Connexin32, a member of the family of proteins that forms gap junction channels between
cells, was immunoaffinity-purified from rat liver using a monoclonal antibody, under …

The ability of certain connexins to form open hemichannels has been exploited to study the
pore structure of gap junction (hemi) channels. Cysteine scanning mutagenesis was applied …

A procedure for rapidly determining the functionality of gap junctions constructed of
recombinant connexins in communication-deficient HeLa cells is described. Nuclear …

Guinea‐pig liver gap junctions are constructed from approximately equal amounts of
connexins 26 and 32. The assembly of these connexins into connexon hemichannels and …