Identification of GPC2 as an oncoprotein and candidate immunotherapeutic target in high-risk neuroblastoma

KR Bosse, P Raman, Z Zhu, M Lane, D Martinez… - Cancer cell, 2017 - cell.com
We developed an RNA-sequencing-based pipeline to discover differentially expressed cell-
surface molecules in neuroblastoma that meet criteria for optimal immunotherapeutic target …

Immunotherapeutic targeting of GPC3 in pediatric solid embryonal tumors

MV Ortiz, SS Roberts, J Glade Bender, N Shukla… - Frontiers in …, 2019 - frontiersin.org
Glypican 3 (GPC3) is a heparan sulfate proteoglycan and cell surface oncofetal protein
which is highly expressed on a variety of pediatric solid embryonal tumors including the …

Therapeutically targeting glypican-2 via single-domain antibody-based chimeric antigen receptors and immunotoxins in neuroblastoma

N Li, H Fu, SM Hewitt… - Proceedings of the …, 2017 - National Acad Sciences
Neuroblastoma is a childhood cancer that is fatal in almost half of patients despite intense
multimodality treatment. This cancer is derived from neuroendocrine tissue located in the …

Illuminating the Onco-GPCRome: Novel G protein–coupled receptor-driven oncocrine networks and targets for cancer immunotherapy

V Wu, H Yeerna, N Nohata, J Chiou… - Journal of Biological …, 2019 - ASBMB
G protein–coupled receptors (GPCRs) are the largest gene family of cell membrane–
associated molecules mediating signal transmission, and their involvement in key …

[HTML][HTML] GPC2-CAR T cells tuned for low antigen density mediate potent activity against neuroblastoma without toxicity

S Heitzeneder, KR Bosse, Z Zhu, D Zhelev… - Cancer Cell, 2022 - cell.com
Pediatric cancers often mimic fetal tissues and express proteins normally silenced
postnatally that could serve as immune targets. We developed T cells expressing chimeric …

GPC2 is a potential diagnostic, immunological, and prognostic biomarker in pan-cancer

G Chen, D Luo, N Zhong, D Li, J Zheng, H Liao… - Frontiers in …, 2022 - frontiersin.org
Background Glypican 2 (GPC2), a member of glypican (GPC) family genes, produces
proteoglycan with a glycosylphosphatidylinositol anchor. It has shown its ascending …

GPCRomics: GPCR expression in cancer cells and tumors identifies new, potential biomarkers and therapeutic targets

PA Insel, K Sriram, SZ Wiley, A Wilderman… - Frontiers in …, 2018 - frontiersin.org
G protein-coupled receptors (GPCRs), the largest family of targets for approved drugs, are
rarely targeted for cancer treatment, except for certain endocrine and hormone-responsive …

Next-generation cancer immunotherapy targeting glypican-3

Y Shimizu, T Suzuki, T Yoshikawa, I Endo… - Frontiers in …, 2019 - frontiersin.org
Glypican-3 (GPC3), a 65 kD protein consisting of 580 amino acids, is a heparan sulfate
proteoglycan bound to the cell membrane by glycosylphosphatidylinositol. This protein is …

[HTML][HTML] CAR T cells targeting tumor-associated exons of glypican 2 regress neuroblastoma in mice

N Li, MB Torres, MR Spetz, R Wang, L Peng, M Tian… - Cell Reports …, 2021 - cell.com
Targeting solid tumors must overcome several major obstacles, in particular, the
identification of elusive tumor-specific antigens. Here, we devise a strategy to help identify …

High-affinity monoclonal antibodies to cell surface tumor antigen glypican-3 generated through a combination of peptide immunization and flow cytometry screening

Y Phung, W Gao, YG Man, S Nagata, M Ho - MAbs, 2012 - Taylor & Francis
Isolating high-affinity antibodies against native tumor antigens on the cell surface is not
straightforward using standard hybridoma procedures. Here, we describe a combination …