Instantaneous isotropic volumetric imaging of fast biological processes

N Wagner, N Norlin, J Gierten, G De Medeiros… - Nature …, 2019 - nature.com
To capture highly dynamic biological processes at cellular resolution is a recurring
challenge in biology. Here we show that combining selective-volume illumination with …

Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning

Z Wang, L Zhu, H Zhang, G Li, C Yi, Y Li, Y Yang… - Nature …, 2021 - nature.com
Light-field microscopy has emerged as a technique of choice for high-speed volumetric
imaging of fast biological processes. However, artifacts, nonuniform resolution and a slow …

[HTML][HTML] High-contrast, synchronous volumetric imaging with selective volume illumination microscopy

TV Truong, DB Holland, S Madaan, A Andreev… - Communications …, 2020 - nature.com
Light-field fluorescence microscopy uniquely provides fast, synchronous volumetric imaging
by capturing an extended volume in one snapshot, but often suffers from low contrast due to …

[HTML][HTML] Virtual-scanning light-field microscopy for robust snapshot high-resolution volumetric imaging

Z Lu, Y Liu, M Jin, X Luo, H Yue, Z Wang, S Zuo… - Nature …, 2023 - nature.com
Abstract High-speed three-dimensional (3D) intravital imaging in animals is useful for
studying transient subcellular interactions and functions in health and disease. Light-field …

Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0

V Voleti, KB Patel, W Li, C Perez Campos… - Nature …, 2019 - nature.com
The limited per-pixel bandwidth of most microscopy methods requires compromises
between field of view, sampling density and imaging speed. This limitation constrains …

Deep learning-enhanced light-field imaging with continuous validation

N Wagner, F Beuttenmueller, N Norlin, J Gierten… - Nature …, 2021 - nature.com
Visualizing dynamic processes over large, three-dimensional fields of view at high speed is
essential for many applications in the life sciences. Light-field microscopy (LFM) has …

Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination

TA Planchon, L Gao, DE Milkie, MW Davidson… - Nature …, 2011 - nature.com
A key challenge when imaging living cells is how to noninvasively extract the most
spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane …

[HTML][HTML] Real-time in vivo imaging of the beating mouse heart at microscopic resolution

S Lee, C Vinegoni, PF Feruglio, L Fexon… - Nature …, 2012 - nature.com
Real-time imaging of moving organs and tissues at microscopic resolutions represents a
major challenge in studying the complex biology of live animals. Here we present a …

Whole-animal functional and developmental imaging with isotropic spatial resolution

RK Chhetri, F Amat, Y Wan, B Höckendorf… - Nature …, 2015 - nature.com
Imaging fast cellular dynamics across large specimens requires high resolution in all
dimensions, high imaging speeds, good physical coverage and low photo-damage. To meet …

Imaging the beating heart in the mouse using intravital microscopy techniques

C Vinegoni, AD Aguirre, S Lee, R Weissleder - Nature protocols, 2015 - nature.com
Real-time microscopic imaging of moving organs at single-cell resolution represents a major
challenge in studying complex biology in living systems. Motion of the tissue from the …