Instantaneous isotropic volumetric imaging of fast biological processes
N Wagner, N Norlin, J Gierten, G De Medeiros… - Nature …, 2019 - nature.com
To capture highly dynamic biological processes at cellular resolution is a recurring
challenge in biology. Here we show that combining selective-volume illumination with …
challenge in biology. Here we show that combining selective-volume illumination with …
Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning
Z Wang, L Zhu, H Zhang, G Li, C Yi, Y Li, Y Yang… - Nature …, 2021 - nature.com
Light-field microscopy has emerged as a technique of choice for high-speed volumetric
imaging of fast biological processes. However, artifacts, nonuniform resolution and a slow …
imaging of fast biological processes. However, artifacts, nonuniform resolution and a slow …
[HTML][HTML] High-contrast, synchronous volumetric imaging with selective volume illumination microscopy
Light-field fluorescence microscopy uniquely provides fast, synchronous volumetric imaging
by capturing an extended volume in one snapshot, but often suffers from low contrast due to …
by capturing an extended volume in one snapshot, but often suffers from low contrast due to …
[HTML][HTML] Virtual-scanning light-field microscopy for robust snapshot high-resolution volumetric imaging
Abstract High-speed three-dimensional (3D) intravital imaging in animals is useful for
studying transient subcellular interactions and functions in health and disease. Light-field …
studying transient subcellular interactions and functions in health and disease. Light-field …
Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0
V Voleti, KB Patel, W Li, C Perez Campos… - Nature …, 2019 - nature.com
The limited per-pixel bandwidth of most microscopy methods requires compromises
between field of view, sampling density and imaging speed. This limitation constrains …
between field of view, sampling density and imaging speed. This limitation constrains …
Deep learning-enhanced light-field imaging with continuous validation
N Wagner, F Beuttenmueller, N Norlin, J Gierten… - Nature …, 2021 - nature.com
Visualizing dynamic processes over large, three-dimensional fields of view at high speed is
essential for many applications in the life sciences. Light-field microscopy (LFM) has …
essential for many applications in the life sciences. Light-field microscopy (LFM) has …
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
A key challenge when imaging living cells is how to noninvasively extract the most
spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane …
spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane …
[HTML][HTML] Real-time in vivo imaging of the beating mouse heart at microscopic resolution
Real-time imaging of moving organs and tissues at microscopic resolutions represents a
major challenge in studying the complex biology of live animals. Here we present a …
major challenge in studying the complex biology of live animals. Here we present a …
Whole-animal functional and developmental imaging with isotropic spatial resolution
Imaging fast cellular dynamics across large specimens requires high resolution in all
dimensions, high imaging speeds, good physical coverage and low photo-damage. To meet …
dimensions, high imaging speeds, good physical coverage and low photo-damage. To meet …
Imaging the beating heart in the mouse using intravital microscopy techniques
Real-time microscopic imaging of moving organs at single-cell resolution represents a major
challenge in studying complex biology in living systems. Motion of the tissue from the …
challenge in studying complex biology in living systems. Motion of the tissue from the …