CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …

Genome editing with engineered nucleases, a powerful tool for understanding biological
function and revealing causality, was built in a joint effort by academia and industry in 1994 …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

The prokaryotic CRISPR-Cas adaptive immune systems provide valuable resources to
develop genome editing tools, such as CRISPR-Cas9 and CRISPR-Cas12a/Cpf1. Recently …

CRISPR/Cas9 is an effective gene editing tool with broad applications for the prevention or
treatment of numerous diseases. It depends on CRISPR (clustered regularly interspaced …

Owing to its easy-to-use and multiplexing nature, the genome editing tool CRISPR-Cas9
(clustered regularly interspaced short palindromic repeats (CRISPR) associated nuclease 9) …

With the introduction of precision genome editing using CRISPR–Cas9 technology, we have
entered a new era of genetic engineering and gene therapy. With RNA-guided …

CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-
programmed target site, limiting their sequence accessibility for robust genome editing …

The rapid development of genome editing technology has brought major breakthroughs in
the fields of life science and medicine. In recent years, the clustered regularly interspaced …

Genome editing is the modification of genomic DNA at a specific target site in a wide variety
of cell types and organisms, including insertion, deletion and replacement of DNA, resulting …