Efficient genotype‐independent cotton genetic transformation and genome editing

X Ge, J Xu, Z Yang, X Yang, Y Wang… - Journal of Integrative …, 2023 - Wiley Online Library
Cotton (Gossypium spp.) is one of the most important fiber crops worldwide. In the last two
decades, transgenesis and genome editing have played important roles in cotton …

A Stable Agrobacterium rhizogenes-Mediated Transformation of Cotton (Gossypium hirsutum L.) and Plant Regeneration From Transformed Hairy Root via …

ML Cui, C Liu, CL Piao, CL Liu - Frontiers in Plant Science, 2020 - frontiersin.org
Genetic transformation is a powerful tool to study gene function, secondary metabolism
pathways, and molecular breeding in crops. Cotton (Gossypium hirsutum L.) is one of the …

Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.)

VN Juturu, GK Mekala, PB Kirti - Plant Cell, Tissue and Organ Culture …, 2015 - Springer
Cotton (Gossypium spp.) is an economically very important fiber yielding crop, which is
grown almost in sixty-five countries throughout the world. Like other crops, cotton also …

Tissue culture-independent in planta transformation strategy: an Agrobacterium tumefaciens-mediated gene transfer method to overcome recalcitrance in cotton …

E Keshamma, S Rohini, K Sankara Rao… - Journal of cotton …, 2008 - repository.ias.ac.in
The major constraint in cotton improvement has been the recalcitrance of cotton varieties to
tissue culture. Alternate methods that avoid/minimize tissue culture would be beneficial for …

A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.): Embryogenic calli as a source to generate large numbers of …

S Leelavathi, VG Sunnichan, R Kumria, GP Vijaykanth… - Plant cell reports, 2004 - Springer
A protocol is presented for efficient transformation and regeneration of cotton. Embryogenic
calli co-cultivated with Agrobacterium carrying cry1Ia5 gene were cultured under …

A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

H Li, K Li, Y Guo, J Guo, K Miao, JR Botella, CP Song… - Plant Methods, 2018 - Springer
Background Genetically modified cotton accounts for 64% of the world's cotton growing area
(22.3 million hectares). The genome sequencing of the diploid cotton progenitors …

High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated …

T Khan, VS Reddy, S Leelavathi - Plant Cell, Tissue and Organ Culture …, 2010 - Springer
A highly efficient and reproducible regeneration system based on somatic embryogenesis in
Gossypium hirsutum cv. Narasimha (NM), which has superior fiber qualities and is also used …

Highly efficient targeted gene editing in upland cotton using the CRISPR/Cas9 system

S Zhu, X Yu, Y Li, Y Sun, Q Zhu, J Sun - International journal of molecular …, 2018 - mdpi.com
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) gene editing system has been shown to be able to induce highly efficient …

Agrobacterium-mediated transformation and regeneration of cotton.

TA Wilkins, R Mishra, NL Trolinder - 2004 - cabidigitallibrary.org
The development of transgenic cotton (Gossypium hirsutum) requires an efficient means for
the transformation and regeneration of fertile plants. Despite the commercial success of …

Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton via Efficient Selection and Timely Subculture of Somatic Embryos

SJ Wu, HH Wang, FF Li, TZ Chen, J Zhang… - Plant Molecular Biology …, 2008 - Springer
Agrobacterium-tumefaciens-mediated transformation of cotton embryogenic calli (EC) was
enhanced by choosing appropriate EC and improving efficiency of coculture, selection …