Optimized exosome isolation protocol for cell culture supernatant and human plasma
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and
prognosis of disease. However, there is currently limited information elucidating the most …
prognosis of disease. However, there is currently limited information elucidating the most …
A comparative study of serum exosome isolation using differential ultracentrifugation and three commercial reagents
Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating
exosomes with reliable quality and substantial concentration is a major challenge. Our …
exosomes with reliable quality and substantial concentration is a major challenge. Our …
Exosome isolation by ultracentrifugation and precipitation and techniques for downstream analyses
C Coughlan, KD Bruce, O Burgy… - Current protocols in …, 2020 - Wiley Online Library
Abstract Exosomes are 50‐to 150‐nm‐diameter extracellular vesicles secreted by all
mammalian cells except mature red blood cells and contribute to diverse physiological and …
mammalian cells except mature red blood cells and contribute to diverse physiological and …
Advances in exosomes technology
Exosomes, also called extracellular vesicles (EVs), are membranous structures measuring
between 40 and 100 nm. Exosomes, secreted by various cells of the human body into body …
between 40 and 100 nm. Exosomes, secreted by various cells of the human body into body …
A comparison of traditional and novel methods for the separation of exosomes from human samples
LL Yu, J Zhu, JX Liu, F Jiang, WK Ni… - BioMed research …, 2018 - Wiley Online Library
Exosomes are discrete populations of small (40‐200 nm in diameter) membranous vesicles
that are released into the extracellular space by most cell types, eventually accumulating in …
that are released into the extracellular space by most cell types, eventually accumulating in …
Comparison of an optimized ultracentrifugation method versus size-exclusion chromatography for isolation of exosomes from human serum
M An, J Wu, J Zhu, DM Lubman - Journal of proteome research, 2018 - ACS Publications
Exosomes are nanosized vesicles that are abundant in biological fluids. In recent years,
exosomes have attracted increasing attention as their cargo may provide promising …
exosomes have attracted increasing attention as their cargo may provide promising …
Benchtop isolation and characterization of functional exosomes by sequential filtration
Early and minimally invasive detection of malignant events or other pathologies is of utmost
importance in the pursuit of improved patient care and outcomes. Recent evidence indicates …
importance in the pursuit of improved patient care and outcomes. Recent evidence indicates …
A protocol for exosome isolation and characterization: evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods
Exosomes are 40–150 nm extracellular vesicles that are released from a multitude of cell
types, and perform diverse cellular functions including intercellular communication, antigen …
types, and perform diverse cellular functions including intercellular communication, antigen …
Review on strategies and technologies for exosome isolation and purification
J Chen, P Li, T Zhang, Z Xu, X Huang… - … in bioengineering and …, 2022 - frontiersin.org
Exosomes, a nano-sized subtype of extracellular vesicles secreted from almost all living
cells, are capable of transferring cell-specific constituents of the source cell to the recipient …
cells, are capable of transferring cell-specific constituents of the source cell to the recipient …
Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived …
Exosomes are 40–100nm extracellular vesicles that are released from a multitude of cell
types, and perform diverse cellular functions including intercellular communication, antigen …
types, and perform diverse cellular functions including intercellular communication, antigen …