A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms

CG Pierce, P Uppuluri, S Tummala… - JoVE (Journal of …, 2010 - jove.com
Candida albicans remains the most frequent cause of fungal infections in an expanding
population of compromised patients and candidiasis is now the third most common infection …

Techniques for Antifungal Susceptibility Testing of Candida albicans Biofilms

G Ramage, JL López-Rib - Antifungal agents: methods and protocols, 2005 - Springer
Candida albicans is capable of forming biofilms on a variety of inert and biological surfaces.
Cells in biofilms display phenotypic properties that are radically different from their free …

Development of a 96-well catheter-based microdilution method to test antifungal susceptibility of Candida biofilms

EI Nweze, A Ghannoum, J Chandra… - Journal of …, 2012 - academic.oup.com
Background Candida biofilms, which are often associated with device-related infections,
including catheter-related bloodstream infections, are resistant to commonly used antifungal …

Standardized Method for In Vitro Antifungal Susceptibility Testing of Candida albicansBiofilms

G Ramage, K Vande Walle, BL Wickes… - Antimicrobial agents …, 2001 - Am Soc Microbiol
Candida albicans is implicated in many biomaterial-related infections. Typically, these
infections are associated with biofilm formation. Cells in biofilms display phenotypic traits …

Comparative analysis of Candida biofilm quantitation assays

HT Taff, JE Nett, DR Andes - Sabouraudia, 2012 - academic.oup.com
Candida grows on devices producing treatment resistant biofilms. A key tool for the study of
biofilms includes an accurate assessment of viable cell growth. This study systematically …

Development of a High-Throughput Candida albicans Biofilm Chip

A Srinivasan, P Uppuluri, J Lopez-Ribot… - PLoS …, 2011 - journals.plos.org
We have developed a high-density microarray platform consisting of nano-biofilms of
Candida albicans. A robotic microarrayer was used to print yeast cells of C. albicans …

A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing

CG Pierce, P Uppuluri, AR Tristan, FL Wormley Jr… - Nature protocols, 2008 - nature.com
The incidence of fungal infections has increased significantly over the past decades. Very
often these infections are associated with biofilm formation on implanted biomaterials and/or …

In Vitro Culturing and Screening of Candida albicans Biofilms

M Gulati, MB Lohse, CL Ennis… - Current protocols in …, 2018 - Wiley Online Library
Candida albicans is a normal member of the human microbiota that asymptomatically
colonizes healthy individuals, however it is also an opportunistic pathogen that can cause …

Assessment and Optimizations of Candida albicansIn Vitro Biofilm Assays

MB Lohse, M Gulati, A Valle Arevalo… - Antimicrobial agents …, 2017 - Am Soc Microbiol
Candida albicans biofilms have a significant medical impact due to their rapid growth on
implanted medical devices, their resistance to antifungal drugs, and their ability to seed …

Optimizing a Candida biofilm microtiter plate model for measurement of antifungal susceptibility by tetrazolium salt assay

JE Nett, MT Cain, K Crawford… - Journal of clinical …, 2011 - Am Soc Microbiol
Candida spp. infect medical devices, such as venous and urinary catheters, by adhering to
the surface and forming a community of drug-resistant cells surrounded by a matrix. The …