Neisseria gonorrhoeae was cultivated in mammalian cell cultures in an effort to determine if
this environment will elicit a T4→ T1 transition. Of four avirulent (T4) isolates tested, only …

Phagocytosis of Neisseria gonorrhoeae in tissue cell-cultures may protect it from the lethal
action of bactericidal agents. Type reversal from avirulent to virulent gonococci was not …

As a basis for studies of gonococcal pathogenicity, tissue culture cells were infected with
type 1 or type 4 Neisseria gonorrhoeae to determine intracellular viability. A simple and …

Neisseria gonorrhoeae 188, type 1, was cultured with monolayers of Rhesus monkey kidney
(RE2) cells. Attachment of gonococci to RE2 cells occurred from about 2 h after inoculation …

Virulent gonococci produce characteristic colony types on solid media, easily
distinguishable from colonies arising from avirulent organisms [1 1. It is possible, therefore …

Neisseria gonorrhoeae was cultivated in a human diploid cell strain (WI-38). Eighty percent
of the cultures contained viable gonococci for at least 4 m at 36 C, as evidenced by …

Despite substantial improvements in solid culture media for gonococci and the development
of liquid media for the short-term growth of the organism, we know of no study of the long …

Gonococci (strain bs3) passaged three times and harvested directly from plastic chambers
implanted subcutaneously in guinea pigs were compared with the parent strain (bs) grown …

Gonococci from subcutaneously implanted chambers in guinea pigs produced, on agar,
more than 95% small colonies showing a 'double highlight'(DH) effect in oblique reflected …

The extensive in vitro cultivation methods used in propagating and maintaining gonococcal
cells were found to affect their virulence, antigenicity, and ultrastructure. Adapting a …