Understanding the behavior of transgenes introduced into oocytes or embryos is essential
for evaluating the methodologies for transgenic animal production. We investigated the …

Intracytoplasmic sperm injection (ICSI)-mediated gene transfer has recently been shown to
be an effective technique for producing transgenic pigs; however, the types of sperm …

The objective of this study was to examine whether the ICSI-mediated gene transfer method
using in vitro matured oocytes and frozen sperm head could actually produce transgenic …

Although pronuclear DNA micro-injection has long been the most reliable method to
produce transgenic pigs, the efficiency of production of transgenic offspring is generally …

The pig is one of most important and valuable animals as livestock for not only meat
producers but also medical use such as organ donors for X/ENotransplantation and as …

As a method of producing transgenic animals, spermatozoa have been used to fertilize
mammalian oocytes through natural copulation, artificial insemination (AI), and in vitro …

Genetically modified domestic animals have many potential applications ranging from basic
research to production agriculture. One of the goals in transgenic animal production …

Transgenesis is an essential tool in many biotechnological applications. Intracytoplasmic
sperm injection (ICSI)-mediated gene transfer is a powerful technique to obtain transgenic …

An efficient and low‐cost production of transgenic pigs has significant applications to the pig
industry and biomedical science. Generation of transgenic pig by sperm‐mediated gene …

The effect of timing of microinjection of DNA constructs on the efficiency of transgenic
embryo production and improved efficiency and quality through combining EGFP as a …