Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440

GJ Bentley, N Narayanan, RK Jha, D Salvachúa… - Metabolic …, 2020 - Elsevier
Pseudomonas putida KT2440 has received increasing attention as an important biocatalyst
for the conversion of diverse carbon sources to multiple products, including the olefinic …

Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering

C Ling, GL Peabody, D Salvachúa, YM Kim… - Nature …, 2022 - nature.com
Muconic acid is a bioprivileged molecule that can be converted into direct replacement
chemicals for incumbent petrochemicals and performance-advantaged bioproducts. In this …

Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity

CW Johnson, D Salvachúa, P Khanna, H Smith… - Metabolic engineering …, 2016 - Elsevier
The conversion of biomass-derived sugars and aromatic molecules to cis, cis-muconic acid
(referred to hereafter as muconic acid or muconate) has been of recent interest owing to its …

Bioprocess development for muconic acid production from aromatic compounds and lignin

D Salvachúa, CW Johnson, CA Singer, H Rohrer… - Green …, 2018 - pubs.rsc.org
Muconic acid (MA) is a bio-based platform chemical that can be converted into the
commodity petrochemical building blocks adipic acid or terephthalic acid, or used in …

Biosensor‐Enabled Directed Evolution to Improve Muconic Acid Production in Saccharomyces cerevisiae

JM Leavitt, JM Wagner, CC Tu, A Tong… - Biotechnology …, 2017 - Wiley Online Library
Muconic acid is a valuable platform chemical with potential applications in the production of
polymers such as nylon and polyethylene terephthalate (PET). The conjugate base …

A novel muconic acid biosynthesis approach by shunting tryptophan biosynthesis via anthranilate

X Sun, Y Lin, Q Huang, Q Yuan… - Applied and environmental …, 2013 - Am Soc Microbiol
Muconic acid is the synthetic precursor of adipic acid, and the latter is an important platform
chemical that can be used for the production of nylon-6, 6 and polyurethane. Currently, the …

Engineering E. coliE. coli cocultures for production of muconic acid from glycerol

H Zhang, Z Li, B Pereira, G Stephanopoulos - Microbial cell factories, 2015 - Springer
Background cis, cis-Muconic acid is an important chemical that can be biosynthesized from
simple substrates in engineered microorganisms. Recently, it has been shown that …

Metabolic engineering of a novel muconic acid biosynthesis pathway via 4-hydroxybenzoic acid in Escherichia coli

S Sengupta, S Jonnalagadda… - Applied and …, 2015 - Am Soc Microbiol
ABSTRACT cis, cis-Muconic acid (MA) is a commercially important raw material used in
pharmaceuticals, functional resins, and agrochemicals. MA is also a potential platform …

Generation of Pseudomonas putida KT2440 Strains with Efficient Utilization of Xylose and Galactose via Adaptive Laboratory Evolution

HG Lim, T Eng, D Banerjee, G Alarcon… - ACS Sustainable …, 2021 - ACS Publications
While Pseudomonas putida KT2440 has great potential for biomass-converting processes,
its inability to utilize the biomass abundant sugars xylose and galactose has limited its …

Functional implementation of a linear glycolysis for sugar catabolism in Pseudomonas putida

A Sánchez-Pascuala, L Fernández-Cabezón… - Metabolic …, 2019 - Elsevier
The core metabolism for glucose assimilation of the soil bacterium and platform strain
Pseudomonas putida KT2440 has been reshaped from the native, cyclically-operating …