Evaluation of different culture systems on the in vitro production of bovine embryos

DC Pereira, MAN Dode, R Rumpf - Theriogenology, 2005 - Elsevier
The objective of this study was to determine the development potential and quality of in vitro
produced bovine embryos cultured individually or in groups. After IVM and IVF, presumptive …

Production of piglets derived from in vitro-produced blastocysts fertilized and cultured in chemically defined media: effects of theophylline, adenosine, and cysteine …

K Yoshioka, C Suzuki, S Itoh, K Kikuchi… - Biology of …, 2003 - academic.oup.com
To further develop defined conditions for in vitro fertilization (IVF) and in vitro culture (IVC) of
in vitro-matured porcine oocytes, we evaluated the effects of theophylline, adenosine, and …

Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature

Q Lin, QA Le, K Takebayashi, M Hirata… - … in Domestic Animals, 2022 - Wiley Online Library
This study developed an efficient method for liquid storage of in vitro‐derived porcine
blastocysts at ambient temperature for 24 hr. We evaluated the effects of new chemically …

Effect of various growth factors in a defined culture medium on in vitro development of bovine embryos matured and fertilized in vitro

ES Lee, Y Fukui - Theriogenology, 1995 - Elsevier
Bovine embryos matured and fertilized in vitro were cultured in a simple, protein-free,
chemically defined medium consisting of synthetic oviduct fluid medium (SOFM) modified by …

Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development

JR Dobrinsky, LA Johnson, D Rath - Biology of reproduction, 1996 - academic.oup.com
Media are available that can deliver modest porcine embryonic development from a single-
cell zygote to the blastocyst stage. However, few embryos develop to hatched blastocysts by …

Blastocyst expansion, hatching, and attachment of porcine embryos cocultured with bovine fibroblasts in vitro

FB Kuzan, RW Wright jr - Animal Reproduction Science, 1982 - Elsevier
The objective of this study was to determine the effect of bovine uterine and testicular
fibroblasts on the in vivo development of porcine morulae and blastocysts. One hundred …

Effects of co-culture, medium components and gas phase on in vitro culture of in vitro matured and in vitro fertilized bovine embryos

H Nakao, N Nakatsuji - Theriogenology, 1990 - Elsevier
To develop an in vitro culture system for bovine oocytes and early embryos, we examined
the effects of co-culture of in vitro matured and in vitro fertilized embryos with trophoblastic …

Culture of in vitro produced bovine zygotes in vitro vs in vivo: implications for early embryo development and quality

BP Enright, P Lonergan, A Dinnyes, T Fair, FA Ward… - Theriogenology, 2000 - Elsevier
The objectives of this study were to examine the effect of culture system on bovine blastocyst
formation rales and quality. Presumptive IVM/IVF bovine zygotes were cultured either in vitro …

Viability of one-cell bovine embryos cultured in vitro: comparison of cell-free culture with co-culture

K Goto, N Iwai, K Ide, Y Takuma, Y Nakanishi - Reproduction, 1994 - rep.bioscientifica.com
In this study, the quality (number of cells) and pregnancy rates of bovine blastocysts
produced by in vitro maturation/in vitro fertilization (IVM/IVF) following cultivation in either …

Adding essential amino acids at a low concentration improves the development of in vitro fertilized porcine embryos

LFS Beebe, I Vassiliev, S Mcilfatrick… - Journal of Reproduction …, 2009 - jstage.jst.go.jp
The aims of this study were to investigate improvements to the pig preimplantation embryo
culture system using in vitro produced embryos. For experiment 1, the optimum time to …