[HTML][HTML] Impact of cryopreservation and freeze-thawing on therapeutic properties of mesenchymal stromal/stem cells and other common cellular therapeutics

C Cottle, AP Porter, A Lipat, C Turner-Lyles… - Current stem cell …, 2022 - Springer
Abstract Purpose of Review Cryopreservation and its associated freezing and thawing
procedures–short “freeze-thawing”–are among the final steps in economically viable …

Cryopreservation of human mesenchymal stem cells for clinical applications: current methods and challenges

KW Yong, WKZ Wan Safwani, F Xu… - Biopreservation and …, 2015 - liebertpub.com
Mesenchymal stem cells (MSCs) hold many advantages over embryonic stem cells (ESCs)
and other somatic cells in clinical applications. MSCs are multipotent cells with strong …

[HTML][HTML] The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review

S Bahsoun, K Coopman, EC Akam - Journal of translational medicine, 2019 - Springer
Mesenchymal stem cells (MSCs) represent an invaluable asset for the field of cell therapy.
Human Bone marrow-derived MSCs (hBM-MSCs) are one of the most commonly used cell …

Off the shelf cellular therapeutics: factors to consider during cryopreservation and storage of human cells for clinical use

EJ Woods, S Thirumala, SS Badhe-Buchanan… - Cytotherapy, 2016 - Elsevier
The field of cellular therapeutics has immense potential, affording an exciting array of
applications in unmet medical needs. One of several key issues is an emphasis on getting …

Technical considerations in the freezing, low-temperature storage and thawing of stem cells for cellular therapies

CJ Hunt - Transfusion Medicine and Hemotherapy, 2019 - karger.com
The commercial and clinical development of cellular therapy products will invariably require
cryopreservation and frozen storage of cellular starting materials, intermediates and/or final …

Cryopreservation of hematopoietic stem cells: emerging assays, cryoprotectant agents, and technology to improve outcomes

K Hornberger, G Yu, D McKenna, A Hubel - Transfusion Medicine and …, 2019 - karger.com
Hematopoietic stem cell (HSC) therapy is widely used to treat a growing number of
hematological and non-hematological diseases. Cryopreservation of HSCs allows for cells …

[HTML][HTML] Mesenchymal stromal cells derived from various tissues: biological, clinical and cryopreservation aspects

LA Marquez-Curtis, A Janowska-Wieczorek… - Cryobiology, 2015 - Elsevier
Originally isolated from bone marrow, mesenchymal stromal cells (MSCs) have since been
obtained from various fetal and post-natal tissues and are the focus of an increasing number …

[HTML][HTML] Cryopreservation as a key element in the successful delivery of cell-based therapies—a review

J Meneghel, P Kilbride, GJ Morris - Frontiers in medicine, 2020 - frontiersin.org
Cryopreservation is a key enabling technology in regenerative medicine that provides stable
and secure extended cell storage for primary tissue isolates and constructs and prepared …

Cryopreserved or fresh mesenchymal stromal cells: only a matter of taste or key to unleash the full clinical potential of MSC therapy?

G Moll, S Geißler, R Catar, L Ignatowicz… - … and cryopreservation of …, 2016 - Springer
Mesenchymal stromal cells (MSCs) harbor great therapeutic potential for numerous
diseases. From early clinical trials, success and failure analysis, bench-to-bedside and back …

Cryopreservation: vitrification and controlled rate cooling

CJ Hunt - Stem cell banking: concepts and protocols, 2017 - Springer
Cryopreservation is the application of low temperatures to preserve the structural and
functional integrity of cells and tissues. Conventional cooling protocols allow ice to form and …