The past decade has witnessed an explosion in the identification of ubiquitin-ligase
complexes as the missing receptors for important small-molecule hormones regulating plant …

Protein ubiquitination requires the concerted action of three enzymes: ubiquitin‐activating
enzyme (E1), ubiquitin‐conjugating enzyme (E2) and ubiquitin ligase (E3). These …

Conjugation of multiple ubiquitins serves as a committed step in the degradation of a variety
of intracellular eukaryotic proteins by the 26S proteasome. Conjugates are formed via a …

Background The ubiquitin 26S/proteasome system (UPS), a serial cascade process of
protein ubiquitination and degradation, is the last step for most cellular proteins. There are …

In this article, we summarize Arabidopsis genes encoding ubiquitin, ubiquitin-activating
enzyme (E1), ubiquitin-conjugating enzymes (E2s) and an additional selected set of proteins …

Protein conjugation with ubiquitin, known as ubiquitination, is a key regulatory mechanism to
control protein abundance, localization, and activity in eukaryotic cells. To identify ubiquitin …

The lack of resolution when studying the many different ubiquitin chain types found in
eukaryotic cells has been a major hurdle to our understanding of their specific roles. We …

Covalent attachment of ubiquitin to other cellular proteins has been implicated in a multitude
of diverse physiological processes in eukaryotes including selective protein degradation …

Ubiquitination modulates nearly all aspects of plant life. Here, we reconstituted the
Arabidopsis thaliana ubiquitination cascade in Escherichia coli using a synthetic biology …

Background Ubiquitination is mediated by the sequential action of at least three enzymes:
the E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme) and E3 (ubiquitin …