[HTML][HTML] Perspectives in flow-based microfluidic gradient generators for characterizing bacterial chemotaxis

CJ Wolfram, GW Rubloff, X Luo - Biomicrofluidics, 2016 - pubs.aip.org
Chemotaxis is a phenomenon which enables cells to sense concentrations of certain
chemical species in their microenvironment and move towards chemically favorable regions …

Bacterial chemotaxis in linear and nonlinear steady microfluidic gradients

T Ahmed, TS Shimizu, R Stocker - Nano letters, 2010 - ACS Publications
Diffusion-based microfluidic devices can generate steady, arbitrarily shaped chemical
gradients without requiring fluid flow and are ideal for studying chemotaxis of free-swimming …

Microfluidics for bacterial chemotaxis

T Ahmed, TS Shimizu, R Stocker - Integrative biology, 2010 - academic.oup.com
Microfluidics is revolutionizing the way we study the motile behavior of cells, by enabling
observations at high spatial and temporal resolution in carefully controlled …

Investigation of bacterial chemotaxis in flow-based microfluidic devices

DL Englert, MD Manson, A Jayaraman - Nature protocols, 2010 - nature.com
The plug-in-pond and capillary assays are convenient methods for measuring attractant and
repellent bacterial chemotaxis. However, these assays do not provide quantitative …

A microfluidic device for quantifying bacterial chemotaxis in stable concentration gradients

DL Englert, MD Manson, A Jayaraman - JoVE (Journal of Visualized …, 2010 - jove.com
Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources
of repellent chemicals. Bacteria constantly monitor the concentration of specific …

[HTML][HTML] A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors

W Shang, CY Tsao, X Luo, M Teodoro, R McKay… - …, 2017 - pubs.aip.org
We have developed a user-friendly microfluidic device for the study of gradient-mediated
bacterial behaviors, including chemotaxis. This device rapidly establishes linear …

A compact microfluidic gradient generator using passive pumping

Y Gao, J Sun, WH Lin, DJ Webb, D Li - Microfluidics and nanofluidics, 2012 - Springer
Creating and maintaining a precise molecular gradient which is stable in space and time are
essential to studies of chemotaxis. This paper describes a simple, compact, and user …

Investigation of bacterial chemotaxis using a simple three-point microfluidic system

H Kim, J Ali, K Phuyal, S Park, MJ Kim - BioChip Journal, 2015 - Springer
A three-point microfluidic system was developed and used to experimentally verify bacterial
chemotaxis with known chemoeffectors. Using pneumatically-controlled micro-valves, the …

Diffusion-based and long-range concentration gradients of multiple chemicals for bacterial chemotaxis assays

M Kim, T Kim - Analytical Chemistry, 2010 - ACS Publications
We present a diffusion-driven and long-range concentration gradient generator that uses
hydrogel as a porous membrane to prevent convection flows but allow the diffusion of cell …

Flow-based microfluidic device for quantifying bacterial chemotaxis in stable, competing gradients

DL Englert, MD Manson… - Applied and environmental …, 2009 - Am Soc Microbiol
Chemotaxis is the migration of cells in gradients of chemoeffector molecules. Although
multiple, competing gradients must often coexist in nature, conventional approaches for …