[HTML][HTML] Perspectives in flow-based microfluidic gradient generators for characterizing bacterial chemotaxis
CJ Wolfram, GW Rubloff, X Luo - Biomicrofluidics, 2016 - pubs.aip.org
Chemotaxis is a phenomenon which enables cells to sense concentrations of certain
chemical species in their microenvironment and move towards chemically favorable regions …
chemical species in their microenvironment and move towards chemically favorable regions …
Bacterial chemotaxis in linear and nonlinear steady microfluidic gradients
Diffusion-based microfluidic devices can generate steady, arbitrarily shaped chemical
gradients without requiring fluid flow and are ideal for studying chemotaxis of free-swimming …
gradients without requiring fluid flow and are ideal for studying chemotaxis of free-swimming …
Microfluidics for bacterial chemotaxis
Microfluidics is revolutionizing the way we study the motile behavior of cells, by enabling
observations at high spatial and temporal resolution in carefully controlled …
observations at high spatial and temporal resolution in carefully controlled …
Investigation of bacterial chemotaxis in flow-based microfluidic devices
DL Englert, MD Manson, A Jayaraman - Nature protocols, 2010 - nature.com
The plug-in-pond and capillary assays are convenient methods for measuring attractant and
repellent bacterial chemotaxis. However, these assays do not provide quantitative …
repellent bacterial chemotaxis. However, these assays do not provide quantitative …
A microfluidic device for quantifying bacterial chemotaxis in stable concentration gradients
DL Englert, MD Manson, A Jayaraman - JoVE (Journal of Visualized …, 2010 - jove.com
Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources
of repellent chemicals. Bacteria constantly monitor the concentration of specific …
of repellent chemicals. Bacteria constantly monitor the concentration of specific …
[HTML][HTML] A simple and reusable bilayer membrane-based microfluidic device for the study of gradient-mediated bacterial behaviors
We have developed a user-friendly microfluidic device for the study of gradient-mediated
bacterial behaviors, including chemotaxis. This device rapidly establishes linear …
bacterial behaviors, including chemotaxis. This device rapidly establishes linear …
A compact microfluidic gradient generator using passive pumping
Creating and maintaining a precise molecular gradient which is stable in space and time are
essential to studies of chemotaxis. This paper describes a simple, compact, and user …
essential to studies of chemotaxis. This paper describes a simple, compact, and user …
Investigation of bacterial chemotaxis using a simple three-point microfluidic system
A three-point microfluidic system was developed and used to experimentally verify bacterial
chemotaxis with known chemoeffectors. Using pneumatically-controlled micro-valves, the …
chemotaxis with known chemoeffectors. Using pneumatically-controlled micro-valves, the …
Diffusion-based and long-range concentration gradients of multiple chemicals for bacterial chemotaxis assays
We present a diffusion-driven and long-range concentration gradient generator that uses
hydrogel as a porous membrane to prevent convection flows but allow the diffusion of cell …
hydrogel as a porous membrane to prevent convection flows but allow the diffusion of cell …
Flow-based microfluidic device for quantifying bacterial chemotaxis in stable, competing gradients
DL Englert, MD Manson… - Applied and environmental …, 2009 - Am Soc Microbiol
Chemotaxis is the migration of cells in gradients of chemoeffector molecules. Although
multiple, competing gradients must often coexist in nature, conventional approaches for …
multiple, competing gradients must often coexist in nature, conventional approaches for …
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