In the mid-1980s, methods for making defined mutations to the genome of living cells were
shown to be applicable in mammalian cells. These methods became known as gene …

This chapter provides general advice for investigators who are planning to generate
transgenic mice by introduction of new DNA into the mouse genome. It focuses mainly on …

One of the most powerful strategies to investigate biology we have as scientists, is the ability
to transfer genetic material in a controlled and deliberate manner between organisms. When …

As the major task of sequencing the human genome is near completion and full complement
of human genes are catalogued, attention will be focused on the ultimate goal: to …

Position effects in animal transgenesis have prevented the reproducible success and limited
the initial expectations of this technique in many biotechnological projects. Historically …

This laboratory manual, published in cooperation with the International Society for
Transgenic Technology (ISTT), provides almost all current methods that can be applied to …

Gene transfer technology provides the ability to genetically manipulate the cells of higher
animals. Gene transfer permits both germline and somatic alterations. Such genetic …

Availability of suitable organs for transplantation remains of major concern and projections
indicate that the problem will continue to increase. Therefore, alternatives to the use of …

The development of methods for cell‐mediated transgenesis, based on somatic cell nuclear
transfer, provides a tremendous opportunity to shape the genetic make‐up of livestock …