Morphological and physiological indicators for screening cell lines with high potential for somatic embryo maturation at an early stage of somatic embryogenesis in …

C Peng, F Gao, H Wang, IN Tretyakova, AM Nosov… - Plants, 2022 - mdpi.com
Many cell lines in the embryogenic callus cannot produce somatic embryos (SEs) even if
they meet the optimal SE maturation culture conditions during conifer somatic …

Flow cytometric and morphological analyses of Pinus pinaster somatic embryogenesis

L Marum, J Loureiro, E Rodriguez, C Santos… - Journal of …, 2009 - Elsevier
An approach combining morphological profiling and flow cytometric analysis was used to
assess genetic stability during the several steps of somatic embryogenesis in Pinus pinaster …

Enhancing initiation and proliferation in radiata pine (Pinus radiata D. Don) somatic embryogenesis through seed family screening, zygotic embryo staging and media …

IA Montalbán, N De Diego, P Moncaleán - Acta physiologiae plantarum, 2012 - Springer
In Pinus spp., initiation of somatic embryogenesis (SE) is influenced by the developmental
stage of immature embryos, the genotype of the parent trees and the formulation of tissue …

Somatic embryogenesis in slash pine (Pinus elliottii) from immature embryos cultured in vitro

SM Jain, N Dong, RJ Newton - Plant science, 1989 - Elsevier
Embryogenic callus development was initiated in cultured immature embryo explants of four
different genotypes of slash pine (Pinus elliottii) on several culture media supplemented with …

Pine somatic embryogenesis using zygotic embryos as explants

GS Pullman, K Bucalo - Plant embryo culture: methods and protocols, 2011 - Springer
Somatic embryogenesis (SE) has the potential to be the lowest-cost method to rapidly
produce large numbers of high-value somatic seedlings with desired characteristics for …

Cotyledonary somatic embryos of Pinus pinaster Ait. most closely resemble fresh, maturing cotyledonary zygotic embryos: biological, carbohydrate and proteomic …

A Morel, JF Trontin, F Corbineau, AM Lomenech… - Planta, 2014 - Springer
Cotyledonary somatic embryos (SEs) of maritime pine are routinely matured for 12 weeks
before being germinated and converted to plantlets. Although regeneration success is highly …

Somatic embryogenesis in Pinus halepensis Mill.: an important ecological species from the Mediterranean forest

IA Montalbán, A Setién-Olarra, CL Hargreaves… - Trees, 2013 - Springer
Pinus halepensis Mill. is a common forest species in the Mediterranean area and it is
important for environmental conservation. This study established a method of regenerating …

Somatic embryos can be induced from the vegetative shoot apex of mature Pinus patula trees

RB Malabadi, J Van Staden, CH Bornman - South African Journal of …, 2003 - Elsevier
Embryogenic cultures were initiated and established and somatic embryos produced in
Pinus patula using thin sections from the dome of the vegetative shoot apex. Factors …

[PDF][PDF] International effort to induce somatic embryogenesis in adult pine trees

JF Trontin, T Aronen, C Hargreaves… - … propagation of forest …, 2016 - academia.edu
The genus Pinus includes several species that are economically important and planted
outside their natural ranges as plantation species. Somatic embryogenesis (SE), a …

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

IA Montalbán, N De Diego, E Aguirre Igartua… - Plant Biotechnology …, 2011 - Springer
This study describes for the first time in Pinus genus a plant regeneration system via a
combined pathway of somatic embryogenesis and organogenesis from immature seeds of …