Development and quality of porcine embryos in different culture system and embryo-producing methods

SA Ock, SL Lee, JG Kim, BM Kumar… - Zygote, 2007 - cambridge.org
In this study, the developmental ability and cellular composition of porcine IVF, parthenote
and somatic cell nuclear transfer (SCNT) embryos were evaluated following different in vitro …

Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pigs

K Yamanaka, S Sugimura, T Wakai… - … of Reproduction and …, 2009 - jstage.jst.go.jp
We evaluated the developmental competence of somatic cell nuclear transfer (SCNT)
embryos using in vitro embryo culture systems. Embryos were cultured in NCSU-23, NCSU …

Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1. 2/G2. 2 sequential medium

JE Swain, CL Bormann, RL Krisher - Theriogenology, 2001 - Elsevier
Porcine embryo development in vitro is relatively inefficient compared to other domestic
species. Currently, a single culture medium (NCSU23) is the standard for porcine in vitro …

In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres

GS Im, L Lai, Z Liu, Y Hao, D Wax, A Bonk, RS Prather - Theriogenology, 2004 - Elsevier
This study investigated the effect of culture media and gas atmospheres on the development
of porcine nuclear transfer embryos. Oocytes derived from a local abattoir were matured for …

Relationships between oxygen consumption rate, viability, and subsequent development of in vivo–derived porcine embryos

N Sakagami, K Nishida, K Akiyama, H Abe, H Hoshi… - Theriogenology, 2015 - Elsevier
Oxygen consumption rate of in vivo–derived porcine embryos was measured, and its value
as an objective method for the assessment of embryo quality was evaluated. Embryos were …

Relationship between developmental ability and cell number of day 2 porcine embryos produced by parthenogenesis or somatic cell nuclear transfer

SJ Uhm, MK Gupta, HJ Chung, JH Kim… - … journal of animal …, 2009 - koreascience.kr
In vitro produced porcine embryos have potential application in reproductive biotechnology.
However, their development potential has been very low. This study evaluated the in vitro …

Sexual maturity and reproductive phase of oocyte donor influence the developmental ability and apoptosis of cloned and parthenogenetic porcine embryos

MK Gupta, SJ Uhm, HT Lee - Animal reproduction science, 2008 - Elsevier
This study investigated the influence of the sexual maturity and reproductive phase of oocyte
donor on the developmental ability and quality of porcine embryos produced by somatic cell …

Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro

DB Koo, NH Kim, HT Lee, KS Chung - Theriogenology, 1997 - Elsevier
The objective of this study was to determine the effects of fetal calf serum (FCS), non-
essential MEM amino acids, MEM vitamins and insulin on blastocoel formation, expansion …

Improved in vitro development of porcine embryos with different energy substrates and serum

H Kim, G Lee, S Hyun, S Lee, D Nam, Y Jeong, S Kim… - Theriogenology, 2004 - Elsevier
The effect of replacing 5.5 mM glucose in North Carolina State University (NCSU)-23
medium with 0.5 mM pyruvate/5.0 mM lactate on porcine IVF embryo development was …

The effect of oxygen tension on porcine embryonic development is dependent on embryo type

PJ Booth, P Holm, H Callesen - Theriogenology, 2005 - Elsevier
Reducing oxygen concentration from atmospheric levels during in vitro culture generally, but
not invariably, improves embryonic development across a range of species. Since the few …